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3D Cell Culture

We offer a portfolio of assays for assessment of cellular health and functional performance that have been optimized and confirmed to work with many 3D model systems. Assays and protocols are optimized to ensure the reagents effectively lyse 3D structures, the reagents effectively penetrate to the center of 3D spheroids, and the mass of cells does not quench the assay signal before it is detected.

Save 20% on Promega's cell health assays validated & optimized for 3D cell culture. Learn more.

13 Products


Assay Readout


Assay Type


Cell State/Activity Detected

Shop all 3D Cell Culture

LDH-Glo™ Cytotoxicity Assay

Sensitive detection of cytotoxicity for samples low in cell number, including 3D microtissue cultures.

J2380, J2381

Autophagy Assay

A no-wash, luminometer-based assay to quantify autophagic flux.

GA1040, GA1050, GA2550

RealTime-Glo™ Apoptosis and Necrosis Assay

A one-step, plate-based assay for continually monitoring apoptosis progression.

JA1011, JA1012, JA1000, JA1001

Glucose-Glo™ Assay

Easily detect glucose from a variety of biological samples.

J6021, J6022

Lactate-Glo™ Assay

Bioluminescent assay to quickly detect lactate from a variety of sample types.

J5021, J5022

Glutamate-Glo™ Assay

Quickly measure glutamate from many sample types.

J7021, J7022

Glutamine/Glutamate-Glo™ Assay

Detect glutamine and glutamate in biological samples.

J8021, J8022

RealTime-Glo™ MT Cell Viability Assay

A bioluminescent method to kinetically monitor viability in cell culture up to 72 hours.

G9711, G9712, G9713

CellTiter-Glo® 3D Cell Viability Assay

A homogeneous method optimized to assess viability in 3D cell culture.

G9681, G9682, G9683

CellTox™ Green Cytotoxicity Assay

An assay to kinetically monitor cytotoxicity up to 72 hours with multiplex capability.

G8741, G8742, G8743, G8731

GSH/GSSG-Glo™ Assay

Homogeneous assay to quantify total glutathione and glutathione ratios.

V6611, V6612

GSH-Glo™ Glutathione Assay

Homogeneous, bioluminescent assay to measure glutathione.

V6911, V6912

Caspase-Glo® 3/7 Assay Systems

Measure caspase-3/7 activities with this homogeneous, luminescent assay.

G8090, G8091, G8093, G8092

Showing 13 of 13 Products