We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

GoTaq® Probe qPCR and RT-qPCR Systems

Convenient Master Mixes for Probe-Based Real-Time qPCR and RT-qPCR

  • Ready-to-use mix of optimized components for robust qPCR and RT-qPCR using hydrolysis probes
  • Compatible with both fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons
  • Resistant to most PCR inhibitors

Choose a system

Size

Catalog number selected: A6101

$ 115.00 Your price:

Add to Cart
This product is discontinued
Add to Helix Add to Helix Add to Helix Add to Helix
GoTaq® Probe qPCR and RT-qPCR Systems
qPCR/2ml
$ 115.00
Your price: Log in
Change Configuration

Simple, Sensitive Probe-Based qPCR and RT-qPCR

The GoTaq® Probe qPCR and RT-qPCR Systems are ready-to-use 2X master mixes that simplify reaction assembly for qPCR using hydrolysis probe detection. These systems are designed for sensitive detection and quantification of a broad range of DNA or RNA targets in the presence of a wide range of PCR inhibitors.

Rapid hot-start activation and processive enzymes make the GoTaq® Probe Systems compatible with most real-time PCR instruments using standard or fast cycling.  Convenient, room-temperature setup makes it easy to scale up to automated and high-throughput detection formats.

Choose qPCR or RT-qPCR Master Mix Formats

GoTaq® Probe qPCR Master Mix
GoTaq® Probe 1-Step RT-qPCR System
GoTaq® Probe 2-Step RT-qPCR System

Sensitivity and Specificity for qPCR

The GoTaq® Probe qPCR Master Mix is a ready-to-use, stabilized, 2X formulation with all of the components for quantitative PCR (qPCR). Just add template, primers and probe. Optimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format, the master mix contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.

The GoTaq® Probe qPCR Master Mix offers:

  • Sensitive detection for earlier quantitation of low- and high-copy targets over a broad dynamic range.
  • Optimized formulation compatible with running multiplex assays.
  • Room-temperature setup, making it suitable for automation and high-throughput detection.
  • Resistance to a wide range of PCR inhibitors.

 

Cat.#Size10µl Reactions20µl Reactions
A6101
2ml
400 reactions
200 reactions
A6102
10ml
2,000 reactions
1,000 reactions
Probe based qPCR from FFPE tissue with GoTaq Master Mix 13203ma

qPCR amplification from FFPE samples with GoTaq® Probe Master Mix. DNA (10ng) from a lung tumor FFPE sample was amplified under both standard and Fast thermal cycling conditions on a Roche LightCycler 480 instrument using a 384-well plate. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

 

Reverse Transcription and qPCR in One Reaction

The GoTaq® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in a single-step real-time amplification reaction.

The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.

Cat.#Size10µl Reactions20µl Reactions
A6120
2ml
400 reactions
200 reactions
Expression levels for the Zmm19 gene in corn by RT-qPCR 13242ma

Expression levels for the Zmm19 gene in corn. Expression was evaluated via 1-step RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120).

 

Two-Step Reverse Transcription and qPCR

The GoTaq® Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix.

The GoTaq® Probe qPCR Master Mix contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

Cat.#Size10µl Reactions20µl Reactions
A6110
2ml
50 × 20µl RT reactions
and 400 × 10µl
qPCR reactions
50 × 20µl RT reactions
and  200 × 20µl
qPCR reactions
Amplification curve for GAPDH detection from human pancreas RNA using the GoTaq® Probe 2-Step RT-qPCR System.

Amplification curve for GAPDH detection from human pancreas RNA using the GoTaq® Probe 2-Step RT-qPCR System.

 

Performance Tested in qPCR with Many Sample Types and Instruments

We have generated experimental data using different systems, instruments, templates, samples and more. Just select the options below to narrow the selection.

 
 

Specifications

You are viewing: A6101 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

GoTaq® Probe qPCR Master Mix, 2X

A610A 2 × 1ml

CXR Reference Dye

C541A 1 × 100μl View Product

Nuclease-Free Water

P119A 2 × 1,250μl View Product

SDS

Choose language:

Certificate of Analysis

See all certificates for A6101 Search
View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Specifications

You are viewing: A6102 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

GoTaq® Probe qPCR Master Mix, 2X

A610A 10 × 1ml

CXR Reference Dye

C541B 2 × 200μl30μM View Product

Nuclease-Free Water

P119E 1 × 13ml View Product

SDS

Choose language:

Certificate of Analysis

See all certificates for A6102 Search
View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Specifications

You are viewing: A6120 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

GoTaq® Probe Master Mix with dUTP

A612A 2 × 1ml

CXR Reference Dye

C541B 1 × 200μl30μM View Product

GoScript™ RT Mix for 1-Step RT-qPCR

M700A 1 × 225μl50X

Nuclease-Free Water

P119A 2 × 1,250μl View Product

SDS

Choose language:

Certificate of Analysis

See all certificates for A6120 Search
View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Specifications

You are viewing: A6110 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 1 × 750μl25mM View Product

GoScript™ 5X Reaction Buffer

A500B 1 × 300μl View Product

GoScript™ Reverse Transcriptase

A501B 1 × 50μl

GoTaq® Probe qPCR Master Mix, 2X

A610A 2 × 1ml

Oligo(dT)15 Primer

C110B 1 × 50μg500μg/ml View Product

PCR Nucleotide Mix

C114G 1 × 200μl10mM View Product

Random Primers

C118B 1 × 50μg View Product

CXR Reference Dye

C541A 1 × 100μl View Product

Recombinant RNasin® Ribonuclease Inhibitor

N251A 1 × 2,500u40u/μl

Nuclease-Free Water

P119A 3 × 1,250μl View Product

SDS

Choose language:

Certificate of Analysis

See all certificates for A6110 Search
View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Choose your country

Americas

Brazil
Canada
United States

Pacific Asia

Australia
India
Japan
Korea, Republic of
Singapore

Europe

Austria
Belgium
Denmark
Estonia
Finland
France
Germany
Iceland
Italy
Luxembourg
Netherlands
Norway
Poland
Spain
Sweden
Switzerland
United Kingdom