HaloTag® Technology: A Powerful Tool for Protein Labeling and Analysis
Start by adding the HaloTag® protein fusion tag to your protein, pair the fusion protein with one of many interchangeable ligands that forms a highly specific covalent bond with HaloTag, and you open a gateway to investigate protein function. Once your protein of interest is fused to the HaloTag® protein, you are ready for numerous applications including protein purification, protein:protein or protein:DNA interactions, cellular imaging, receptor internalization and more. Each possible analysis pathway is based on which interchangeable HaloTag® ligand you select, or customize a ligand for your specific requirements.
Protein Purification from E. coli and Mammalian Cells
Learn how to purify your protein fused to HaloTag from live cells and cell lysates.
Protein Localization, Imaging, Trafficking and Turnover
Track where proteins are in the cell, where they are going and how long they stay using fixed and live cells.
Protein:Protein and Protein:DNA Interaction Analysis
Study how proteins and protein complexes interact in live cells or examine DNA:protein interactions without antibodies.
Super Resolution Microscopy
Follow a single molecule in cells when using a bright Janelia Fluor® ligand with a HaloTag® fusion protein.
Thousands of clones that have been validated by:
- Fluorescent imaging
- DNA sequencing
- Protein expression