Live-cell kinetic assays are detection reagents that allow the same sample well to be repeatedly measured over multiple time points. This saves you time and effort, enabling you to collect more informative data in real time. Learn how you can get better quantitative data without cumbersome multi-plate setups.
Learn How These Assays Work
We provide a portfolio of individual live-cell kinetic assay kits. All of these assays allow you to read the same sample plate over multiple time points. The workflow varies based on the different reagent chemistry.
Example Workflow #1: Repeated Reading from Same Well
Plate your cells and add the reagent once. Then read the plate at each time point, as many times as you need! Our Cell Viability, Cytotoxicity, Target Engagement, Reporter Expression, Apoptosis, Extracellular ATP, Protein:Protein Interactions and Targeted Protein Degradation assays use this workflow.
Example Workflow #2: Repeated Sampling from Same Well
Plate your cells just once. At each time point, remove a small sample of cell media, add reagent and read. Repeat sampling as many times as you need! Our LDH Release and Energy Metabolism assays use this workflow.
See how our RealTime-Glo™ MT Cell Viability Assay works
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Data obtained using the Nano-Glo® Endurazine™ and Vivazine™ Live Cell Substrates.
"We can get way more data from a single experiment..."
— Dr. Monika Kijanska, Sr. Scientist, InSphero
Learn why Dr. Kijanska's team switched to our LDH-Glo™ Cytotoxicity Assay to assess the kinetics of drug-induced hepatotoxicity in 3D liver microtissues.
Better Data Using Any Multimode Plate Reader
Measuring the same sample plate ensures you are comparing the same number of cells and dose treatment over time.
Get unambiguous, straightforward signal values that you can easily analyze and graph.
You can use any multimode plate reader,like the one you already have in the lab, but our GloMax® Instruments are optimized to run these assays. Learn how you can easily get great 24-hour or multiday kinetic data using GloMax® Instruments in this blog.
Save Precious Samples
Sometimes, they give you a little and expect a lot.
By generating data over time from a single well (instead of one well for each time point), those valuable cells go even further.
Sounds Too Good To Be True... What's the Catch?
Like any new assay, you will have to test and verify real-time assays in your lab. That will take time in the beginning. But the time you'll save in the long run makes it worthwhile. You can find detailed protocols for each individual assay in the technical manuals. And don't worry, our Technical Services team can answer any questions you may have. We're here to help you succeed!
We'll help you figure out if real-time technology is right for you.
Chat with one of our scientists or fill out your information to request a free consult. Our Technical Services Team will be in contact to help you understand how these assays can work for you.
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