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Live-Cell Kinetic Assays for Time Course Analysis

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Are you performing time-course and dose-response experiments?

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Are you working with precious primary or patient cells?

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Are you doing drug screenings?

Live-cell kinetic assays are detection reagents that allow the same sample well to be repeatedly measured over multiple time points. This saves you time and effort, enabling you to collect more informative data in real time. Learn how you can get better quantitative data without cumbersome multi-plate setups.

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Transform Your Time-Course into a One-Plate Experiment

No more separate wells for every time point. One plate setup gets you unlimited data.

Less setup, less cells, less time, less pipetting, less reagents, less... well... everything.

Standard Apoptosis Multi-Plate Method
 

Endpoint Assays vs RealTime Apoptosis Assays

Real-Time Apoptosis Assay
 

Apoptosis progression in single well with annexin V assay 14344ma-w-a

Download this poster to learn how the RealTime-Glo™ Annexin V Apoptosis Assay can be used with various cell models exploring the kinetics of apoptosis.

Learn How These Assays Work

We provide a portfolio of individual live-cell kinetic assay kits. All of these assays allow you to read the same sample plate over multiple time points. The workflow varies based on the different reagent chemistry. 

Example Workflow #1: Repeated Reading from Same Well

Plate your cells and add the reagent once. Then read the plate at each time point, as many times as you need! Our Cell ViabilityCytotoxicityTarget EngagementReporter ExpressionApoptosis, Protein:Protein Interactions and Targeted Protein Degradation assays use this workflow.

realtime-workflow-add-mix-read-2

Example Workflow #2: Repeated Sampling from Same Well

Plate your cells just once. At each time point, remove a small sample of cell media, add reagent and read. Repeat sampling as many times as you need! Our LDH Release and Energy Metabolism assays use this workflow.

realtime-workflow-transfer-medium-2


Learn how the RealTime-Glo™ MT Cell Viability Assay is used to screen for novel cancer drugs

See a list of our kinetic assay kits and search peer-reviewed publications.

View Kits and Citations

No More Missing Time Points

Every second, cellular conditions are changing.

What if you miss that one time point that matters?

With real-time assays, you don't need to guess when things will be interesting. 

Make a real curve! Collect data from however many time points you need, all from a single well.

Multiple Assays in One Sample Well

Get even more data per sample by multiplexing two, or even three assays on a single plate. This will allow you to normalize your data, compare orthogonal methods or provide insight to the mechanism of action.

Pair our real-time assays together for any combination of cell viability, cytotoxicity, apoptosis, metabolite quantification and reporter expression.

Cholesterol/Cholesterol Ester-Glo™ Assay Viability Results
Cholesterol/Cholesterol Ester-Glo™ Assay Cholesterol Results
Cholesterol/Cholesterol Ester-Glo™ Assay Toxicity Results

All three of the above graphs were obtained from a single sample plate! Learn more about the data in the technical manual of the Cholesterol/Cholesterol Ester-Glo™ Assay.

"We can get way more data from a single experiment..."  — Dr. Monika Kijanska, Sr. Scientist, InSphero

Learn why Dr. Kijanska's team switched to our LDH-Glo™ Cytotoxicity Assay to assess the kinetics of drug-induced hepatotoxicity in 3D liver microtissues.

View Article

Better Data Using Any Multimode Plate Reader

Measuring the same sample plate ensures you are comparing the same number of cells and dose treatment over time.

Get unambiguous, straightforward signal values that you can easily analyze and graph.

You can use any multimode plate reader, like the one you already have in the lab, but our GloMax® Instruments are optimized to run these assays.

Save Precious Samples

Sometimes, they give you a little and expect a lot.

By generating data over time from a single well (instead of one well for each time point), those valuable cells go even further.

Inserting a plate to run on the GloMax® Discover

Sounds Too Good To Be True... What's the Catch?

Real-time nonlytic assays are a new technique

It's a New Technique

Our real-time assays use new technology that you may not be familiar with. That's why we encourage you to consult with us to make sure expectations are met. Although these assays may be new to you, they're not new to us. We've provided these assays since 2015, and they have been used in hundreds of publications! Search for citations here.

Higher single assay costs lead to savings overall

Single Kit Cost

The cost for a real-time assay kit might seem higher than what you're used to. However, you're getting more data per well because one real-time assay well can be repeatedly measured for as many time points as you need. If you consider the savings of reagents, plasticware, cells, treatments, time spent on duplicating sample plates... you'll likely save costs.

Real-time nonlytic assays require initial validation time

Verification Time

Like any new assay, you will have to test and verify real-time assays in your lab. That will take time in the beginning. But the time you'll save in the long run makes it worthwhile. You can find detailed protocols for each individual assay in the technical manuals. And don't worry, our Technical Services team can answer any questions you may have. We're here to help you succeed! 

 

Need to convince your PI or lab manager before trying out our kinetic assays?
Show them this datasheet that highlights the benefits!

 

Download Datasheet

We'll help you figure out if real-time technology is right for you.

Chat with one of our scientists or fill out your information to request a free consult. Our Technical Services Team will be in contact to help you understand how these assays can work for you.

We respect your privacy and your information will only be used for this purpose.

Chat with a Scientist