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RealTime-Glo™ Extracellular ATP Assay

Live-Cell Kinetic Assay for Measuring Extracellular ATP

  • Get more data per well with real-time monitoring
  • Just add, mix, measure; no supernatant sampling
  • Ideal for high-throughput applications

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Catalog number selected: GA5010

$ 205.00
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RealTime-Glo™ Extracellular ATP Assay
200 assays
$ 205.00
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Monitor Immunogenic Cell Death in Real Time

The RealTime-Glo™ Extracellular ATP Assay is a bioluminescent assay designed for kinetic monitoring of ATP released from dying, stressed or activated cells. Extracellular ATP can function as a damage associated molecular pattern (DAMP) and is a key biomarker for determining whether a treatment induces immunogenic cell death, a specialized form of cell death that results in an immune response.

The RealTime-Glo™ Extracellular ATP Assay allows you to continually monitor extracellular ATP so you won’t miss crucial time points. It is compatible for use in 384-well plates, making it ideal for high-throughput screening applications.

Simple Protocol for Measuring Extracellular ATP

Set up one plate to collect all the time points you need! First, reconstitute the reagent with cell culture medium and add to cells at the time of treatment. Then, read the luminescent signal continuously for 24 hours or longer using a standard plate-reading luminometer. No supernatant sampling required.

RealTime-Glo Extracellular ATP Assay Workflow

Kinetic Monitoring of Immunogenic Cell Death

Kinetic Monitoring of Immunogenic Cell Death

U937 cells were treated with a dilution of mitoxantrone, an anthracycline shown to induce immunogenic cell death (ICD). RealTime-Glo™ Extracellular ATP Assay Reagent was added, and the assay plate was placed in a plate reader set at 37°C. Luminescence was collected every 10 minutes for 24 hours.

Compatible with 2D and 3D Cell Models 

Compatible with 2D Cell Models
Compatible with 3D Cell Models

HCT-116 cells were grown in monolayer (left) and in spheroids using ultra-low attachment plates (right). Cells were treated with dilutions of staurosporine, and the RealTime-Glo™ Extracellular ATP Assay Reagent was added. The timing for ATP release differs between monolayer and spheroid cell culture, and the reagent is compatible with both cell culture models.

Want to measure ATP as a marker of cell viability?

Learn about the CellTiter-Glo® 2.0 Assay, a stable, single-reagent ATP assay.

Specifications

You are viewing: GA5010 Change Configuration

What's in the box?

Item Part # Size

RealTime-Glo™ Extracellular ATP Reagent Substrate

GA501A 1 × 1 vial

SDS

Choose language:

Download SDSPDF (193 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: GA5011 Change Configuration

What's in the box?

Item Part # Size

RealTime-Glo™ Extracellular ATP Reagent Substrate

GA501B 1 × 1 vial

SDS

Choose language:

Download SDSPDF (194 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: GA5012 Change Configuration

What's in the box?

Item Part # Size

RealTime-Glo™ Extracellular ATP Reagent Substrate

GA501A 10 × 1 vial

SDS

Choose language:

Download SDSPDF (194 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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