GPCR Research for Drug Discovery
G protein-coupled receptors (GPCRs) represent one of the most important classes of drug targets. Discover how to measure the response along each step of the GPCR signaling cascade with easy-to-use, bioluminescence-based assays.
Case Study: University of Nottingham Medical School
A novel approach, using NanoBRET™ and NanoLuc® technology, to study the effects of drugs on a GPCR that recognizes and responds to neurotransmitters.
Lighting Up GPCR Research with Bioluminescent Tagging
A summary of a research publication that used the HiBiT assay to measure surface expression of orexin and prokineticin receptors.
A NanoBRET™ Biosensor for GPCR:G protein Interaction with the Kinetics and Temporal Resolution of Patch Clamping
Review of a study to detect signaling through Gα12/13 proteins, which had previously proved intractable, using NanoBRET™ technology.
Measuring cAMP Levels and Cytotoxicity in a Single Plate Well
Multiplexing of a fluorescent real-time cytotoxicity assay with a bioluminescent cAMP assay, to examine cytotoxic effects through coupling of GPCRs with adenylate cyclase activity.