Lumit™ Technology: Reliable, Automation-Friendly Immunodetection
Lumit™ Technology enables bioluminescence-based detection of specific antigens or antibodies, simplifying immunoassay protocols and speeding time to results. Based on the award-winning NanoBiT® complementation system, Lumit™ Technology powers sensitive and accurate immunoassays for research and clinical applications.
Lumit™ Immunoassays provide a faster, more reliable alternative to ELISAs, eliminating the variability associated with multiple wash steps, and providing the convenience of a simple and sensitive luminescent detection method. Because of these advantages, Lumit™ Immunoassays are easily adaptable to automation for labs processing large numbers of samples.
NanoBiT® Technology is the Basis of the Lumit™ Detection Method
NanoLuc® Binary Technology (NanoBiT) is a protein complementation system based on NanoLuc® luciferase, which consists of two subunits—small bit (SmBiT) and large bit (LgBiT)1,2. These subunits have low affinity for each other and only form an active luciferase enzyme when they come into close proximity. The small size of the NanoBiT® complementation partners minimizes interference with normal protein function, and the bright signal accommodates low, native expression levels.
Antigen Detection
In some Lumit™ Immunoassays, antibodies directed against specific target molecules are chemically labeled with SmBiT and LgBiT. When the two antibodies bind their target, SmBiT and LgBiT come into close proximity to form an active luciferase, generating a luminescence signal in the presence of substrate3.
Pre-built Lumit™ Immunoassays for detection of cytokines, glucagon, insulin, phosphorylated proteins and neonatal Fc receptor binding are available, as well as antibody labeling kits and detection reagents for creating your own assays.
Lumit™ Technology Immunoassay Options
DIY Assays
Antibody labeling kit and detection reagents to build your own immunoassays.
Pre-Built Assays
Kits to detect specific targets including cytokines, insulin, glucagon, neonatal fc receptor binding and phosphorylation activity.
Custom Assays
Consult with our team to develop your own Lumit™ application.
Optimize Your Lumit™ Immunoassay Workflow With Support From Our Automation Experts
Our Field Support Specialists are experts in major liquid handler types, including the Promega Maxprep™ System, Hamilton Microlab STAR® series, Tecan Freedom EVO® and Fluent® series, Eppendorf epMotion® and Beckman Biomek® series. We offer prewritten and customized scripts and in-lab/remote assistance. Let us help optimize your current workflow or discuss automation solutions to fit your lab’s needs.
References
- Hall M.P., Unch J., Binkowski B.F., et al. (2012) Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem Biol. 7(11), 1848-1857. doi:10.1021/cb3002478
- Dixon A.S., Schwinn M.K, Hall M.P., et al. (2016) NanoLuc Complementation Reporter Optimized for Accurate Measurement of Protein Interactions in Cells. ACS Chem Biol. 11(2), 400-408. doi:10.1021/acschembio.5b00753
- Hwang B.B., Engel L., Goueli S.A., Zegzouti H. (2020) A homogeneous bioluminescent immunoassay to probe cellular signaling pathway regulation. Commun Biol. 3:8. doi:10.1038/s42003-019-0723-9