RNA Analysis

RNA analysis products include fluorescent dyes for RNA quantification, in vitro transcription reagents, RT-PCR enzymes and kits, and vectors for generating shRNA for use in RNA interference experiments.

Promega RT-PCR products include standalone reverse transcriptases (AMV, M-MLV and GoScript) and a range of complete kits containing all the required enzymes, buffers and reagents for successful RT-PCR. 

Reagents for in vitro transcription include large-scale RNA production systems (RiboMAX), and systems for generation of RNA probes (Riboprobes). 

RNA is notoriously susceptible to nuclease activity. Promega RNasin Ribonuclease Inhibitor is the gold standard used by researchers worldwide to protect RNA from nuclease degradation.

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Introduction to RNA Analysis

RNA species (messenger RNA, transfer RNA, ribosomal RNA) serve essential functions in translation of genetic information from DNA into proteins. More recently, new roles for microRNA and other small non-coding RNAs in the regulation of gene expression have been described.  RNA analysis research covers a wide range of topics—from large-scale analysis of the transcriptome—the complete set of RNA transcripts encoded by a genome—to studying the actions of individual small noncoding RNAs on specific genes. 

In vitro transcription is the process by which RNA is generated from a DNA template in the lab. In vitro transcription systems are used to generate RNA from DNA templates for use in applications such as in vitro translation, the study of RNA viruses and RNA:protein interaction studies. Riboprobe® Systems are used to prepare single-stranded RNA probes, microgram quantities of defined RNA transcripts, and antisense RNA for use in RNA analysis studies. 

RT-qPCR is an essential technique for analysis of gene expression. RT-qPCR systems are used to detect and monitor the expression of specific RNA targets in real time during an experiment.

RNases (enzymes that degrade RNA) are ubiquitous. Successfully isolating RNA and maintaining its integrity is critical, especially when sensitive downstream applications are used (e.g., RNA-Sequencing). All RNA purification and handling should take place in an RNase-free, RNA-only zone of the lab and gloves should always be worn to prevent accidental contamination. Using an RNase inhibitor can help safeguard your samples from RNase degradation.