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Protein analysis

Protein Purification

Protein purification is fundamental to the study of protein function and involves a series of processes to express, enrich and purify a protein of interest from a complex mixture such as cell lysate. The fastest and most powerful method for this purpose is affinity purification. We offer a range of protein purification kits and reagents for purification of recombinant proteins using affinity tags such as HaloTag, GST- or His-tag. We also offer Magne® Protein G/A Beads, which provide an easy-to-use and rapid method for purification of polyclonal and monoclonal antibodies from serum, ascites fluid and cell culture medium.

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Protein Purification Product Groups

Top Protein Purification Products for Your Lab

Protein Purification Basics

Protein purification is a fundamental step for analyzing individual proteins and protein complexes.  Escherichia coli remains the first choice of many researchers for producing recombinant proteins due to ease of use, rapid cell growth and low cost of culture. Proteins expressed in E. coli can be purified in relatively high quantities, but these proteins, especially eukaryotic proteins, may not exhibit proper protein activity or folding. Cultured mammalian cells offer an alternative option for producing properly folded and functional mammalian proteins with appropriate post-translational modifications.  

To simplify purification, affinity purification tags can be fused to a recombinant protein of interest. Common fusion tags are polypeptides, small proteins or enzymes added to the N- or C-terminus of a recombinant protein. The first step in purification of a recombinant protein is the preparation of the cell lysate or supernatant.  For secreted proteins, minimal supernatant preparation is required, followed by selective binding, washing and elution of the purified protein. After purification the protein may be cleaved with a protease to remove the affinity tag.

We offer a complete portfolio of protein analysis products supported by a growing library of educational resources, including technology guides, on-demand webinars, scientific articles and protocols.

Use the links below to learn more about protein analysis products, or to explore learning resources covering topics such as in vitro protein expression, protein purification methods and live-cell protein interaction analysis.