RNA Therapy
Recent technology advancements have led to several approved RNA-based therapies including those using anti-sense oligonucleotides, small non-coding RNA molecules to modulate gene expression or manipulate cellular processes, as well as the use of messenger RNA (mRNA) to express therapeutic proteins.
From research and development through manufacturing, we offer a broad portfolio of products to support your RNA therapy workflow every step of the way.
mRNA Therapeutics Analysis, Characterization and Manufacturing
From research and development through manufacturing, we offer a broad portfolio of products to support every stage of your mRNA therapeutics development workflow.
DNA Plasmid Creation, Amplification, Purification and Linearization
Template Plasmid DNA Creation
Chemical Buffers and Reagents
Cloning Vector Kits and Components
Restriction Enzymes
Modifying Enzymes
PCR Enzymes and Kits
RNasin® Ribonuclease Inhibitor
Premixed dNTPs
Individual dNTPs
Nucleic Acid Extraction Chemistries and Kits
Template Plasmid DNA Amplification in E. coli
Bacterial Strains and Competent Cells
Template Plasmid DNA Endotoxin Removal/Purification
Plasmid Purification Nucleic Acid Clean-Up
Template Plasmid DNA Linearization
Restriction Enzymes
Modifying Enzymes
mRNA Production and Purification
Cell-Free In Vitro Transcription and 5' Capping
In Vitro Transcription Products
RiboMAX™ Large Scale RNA Production Systems
T7 RiboMAX™ Express Large Scale RNA Production System
Ribo m7G Cap Analog
Digestion of DNA with DNase I
Modifying Enzymes
Purification of RNA Product
RNA Extraction Kits
Nucleic Acid Clean-Up Systems
RNA Bulk Product Analytics
ADCC and ADCP Reporter Bioassays
Custom and cGMP Manufacturing
RNA Analysis Solutions
Explore solutions for every step of your RNA analysis workflow.
RNA Analysis Workflow
Created with BioRender.com
Extraction
Reagents for extraction of total RNA and mRNA from blood, cells, tissues and other sample typesManual Purification Solutions
- PureYield™, ReliaPrep™ and SV Systems provide a choice of kits for single tube or 96-well-plate-based manual purification of total RNA or miRNA
- PolyATract® Systems isolate mRNA from total RNA or crude lysates
Maxwell® Instruments
- Consistent purification in a small, reliable bench-top device
- Automated purification of 1–48 samples
Automated Nucleic Acid Purification for Liquid Handlers
- Automated extraction of diverse sample types and volumes
- Platform-agnostic, customized approach to fit your laboratory's specific needs
Protection
RNasin® Ribonuclease Inhibitor- Maintains RNase inhibitory activity over a wide temperature range
- Inhibits a broad spectrum of eukaryotic RNases over a wide pH range (pH 5–8)
Quantitation
- Fluorescent RNA-binding dye for quantitation of small amounts of RNA (100pg) in solution
- Detection on microplate with the GloMax® Instruments or single-tube fluorometers with the Quantus™ Fluorometer
Amplification
Real-Time PCR
- Ready-to-use mix using hydrolysis probes or BRYT Green® Dye for higher fluorescence efficiency and less PCR inhibition than SYBR® Green I
- Multiplex compatible
- Suitable for automation and high-throughput setup
- Resistant to a wide range of PCR inhibitors
- Compatible with both fast and standard cycling methods
Reverse Transcriptase
- Sensitive transcription of both high-copy and low-copy messages
- Transcribes short and long transcripts and processes through secondary structure
- GoScript™ Reverse Transcriptase is available in a variety of formats, from standalone enzymes to mixes and kits
Sequencing
The ProDye™ Terminator Sequencing System provides an improved method of fluorescent Sanger sequencing using a proprietary thermostable DNA polymerase included in the master mix.
- Simple cycling protocol compatible with a variety of thermal cyclers
- Designed for a wide range of sequencing applications and maximum read lengths
The Spectrum Compact CE System is an integrated and efficient instrument that brings you the independence to perform Sanger sequencing and fragment analysis in your laboratory, under your control and at your convenience.
- Sanger sequencing and fragment analysis
- 32 samples (4 x 8-Well Strip Tubes)
- 4 Capillaries (36cm)
- Easy, wizard-like operation and snap-in consumables
- Integrated, intuitive operational touchscreen
RNAi Screening, siRNA and miRNA Activity
| RNA Analysis |
Product Information |
|---|---|
| siRNA and miRNA Activity |
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| RNAi Screening |
Endogenously Tagged Cell Lines |