:

United States

United States

Choose language: English

Home » Resources »

Promega Webinars

NA_webinarsConcept-image
Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. Most presentations are given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

How to Integrate Cellular Metabolism Assays Into Your Research: Considerations and Challenges

Cell-Based Assays

Tuesday, September 12, 2017

Donna Leippe, PhD

Metabolite detection technologies can pose challenges such as laborious sample preparation and the inability to assay numerous samples rapidly. Additionally, lack of sensitivity and dynamic range prevents detection of metabolites directly in cells in multi-well plates. In this webinar, we describe new bioluminescent metabolite assays and sample processing strategies that enable rapid measurements of key energy metabolites in plate formats and show how the assays can be used to study cancer cell metabolism and activation of T cells.

Bioactivity Assays: Putting the Puzzle Together

Drug Discovery

Tuesday, September 19, 2017

Ulrike Herbrand, PhD

In past years, the requirement for bioactivity testing changed from later phase lot release and stability testing to additional applications like biocomparability testing for follow-on biologics, accelerated stress condition testing and confirmation of successful production scale up. Therefore a suitable bioassay is needed at a very early time point. This webinar will look at a wide variety of therapeutic proteins and illustrate some best practices in method development that results in reliable, reproducible and precise assays, and discuss which assays may be best for your product and development program.

Applications of a Bioluminescent Peptide Tag: Simple, Quantitative Protein Detection Down to Endogenous Levels

Cell-Based Assays

Tuesday, September 26, 2017

Christopher Eggers, PhD

Regulated protein abundance and surface expression are fundamental to normal cellular physiology and dysregulation underlies many disease states. Monitoring changes in protein abundance usually involves labor-intensive, antibody-based methods that yield only semi-quantitative results. This webinar will introduce a bioluminescent tagging system that can quantify proteins in minutes using simple, antibody-free methods. This system offers wide dynamic range and sensitivity compatible with detecting even endogenously expressed proteins when combined with CRISPR gene editing.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

How to Avoid Artificial Non-Enzymatic PTMs During the Peptide Sample Preparation Process

Proteomics

Tuesday, June 13, 2017

Sergei Saveliev, PhD

Non-enzymatic post-translational modifications (PTMs) spontaneously occur in biotherapeutic proteins during manufacturing and storage. These modifications negatively affect efficacy and stability of biotherapeutic proteins. Major non-enzymatic PTMs are deamidation, disulfide bond scrambling and oxidation. These non-enzymatic PTMs are also introduced during protein preparation for peptide mapping and compromise the analysis. In the webinar recording Dr. Saveliev discusses sources of these artificial protein modifications as well as procedural optimizations to suppress these PTMs.

Post-Translational Modification Enzymes: How to Interrogate Key Drug Targets

Cell-Based Assays

Tuesday, April 11, 2017

Hicham Zegzouti, PhD

Post-translational modifications (PTMs), the addition of functional groups to proteins, play a significant role in regulating cellular biology. Examples include phosphorylation, a common mechanism in regulating enzymatic activity, and the addition of carbohydrates to many eukaryotic proteins, which promotes proper folding, improves stability and serves in regulatory functions. A key objective of research and drug discovery surrounding PTMs is to study the regulation of these enzymes and find specific modulators of their activity. Although mass spectrometry, eastern and western blotting detection technologies have been used traditionally, none are amenable to high throughput or as sensitive as bioluminescent detection of nucleotide-based substrates for such enzymes.

Optimizing and Qualifying Reporter Gene Bioassays for Immune Checkpoint Receptors

Drug Discovery

Tuesday, March 28, 2017

Zhijie Jey Cheng, PhD

Reporter gene assays for functional screening and development of antibody drug candidates are gaining popularity due to the significant advantages they offer over traditional methods that rely on human primary immune cells. We will discuss development of a series of MoA-based reporter gene bioassays for antibodies targeting co-inhibitory receptors, co-stimulatory receptors and combination bioassays that target immune checkpoint receptors.

Factors to Consider for Designing and Optimizing Assays Applied to 3D Cultures

Cell-Based Assays

Tuesday, March 14, 2017

Terry Riss, PhD

3D cell culture models present a challenge for using assay chemistries and imaging methods that were originally designed for measuring events from 2D monolayers of cells. In this webinar, we will present factors to consider when designing and optimizing assays applied to 3D cell culture models, case studies describing design of reagents to overcome the limitations inherent with detecting markers from large 3D culture models, and applications of novel real-time detection methods.

MicroRNA Analysis Paired with Novel Cell Health Assays: A Complete Workflow

Genomics

Tuesday, February 28, 2017

Brad Hook, PhD

MicroRNAs (miRNAs) are small-noncoding RNA molecules that regulate gene expression post-transcriptionally. Amplification and overexpression of individual oncomiRs (miRNAs associated with cancer) or genetic loss of tumor suppressor miRNAs are associated with human cancer and are sufficient to drive tumorigenesis in mouse models. A global decrease in miRNA levels has been observed in human cancers and linked to genetic and epigenetic alterations in components of the miRNA biogenesis machinery. Learn more about how to analyze miRNAs in human cancer cells as we present data on a two studies that use new novel assays to monitor cell health and purify total RNA (including miRNAs) from the same cells.

  • Results per page 
  • « 1 2 3 4 5  ... » 
Choose your country

Americas

Brazil
Canada
United States

Pacific Asia

Australia
India
Japan
Korea, Republic of
Singapore

Europe

Austria
Belgium
Denmark
Estonia
Finland
France
Germany
Iceland
Italy
Luxembourg
Netherlands
Norway
Poland
Spain
Sweden
Switzerland
United Kingdom