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Promega Webinars

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. Most presentations are given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

NanoBRET: A Quantitative Technique to Measure Kinase Target Engagement in Live Cells

Cell-Based Assays

Tuesday, January 23, 2018

Matthew Robers, MS

Although a number of extracellular techniques exist to quantitatively measure kinase-ligand binding or inhibition, until now there was no means by which to quantitative analyze target occupancy or drug affinity in live cells. In this webinar we report the use of an energy transfer technique (NanoBRET) in the first quantitative approach to profiling kinase occupancy of a test compound inside live cells.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

Metagenomic Mapping of Medical, Urban and Space Environments

MDx

Tuesday, December 05, 2017

Christopher Mason, PhD

Many new methods in genomics enable an integrative, cross-kingdom view of patients (precision metagenomics) and their environment, including metagenome profiles of the world’s cities (MetaSUB.org) and antimicrobial resistance (AMR) markers. In this webinar, technologies that can sequence, quantify, and map nucleic acids will be covered, for Earth and beyond, focusing on methods from Promega that enable these projects and missions.

Improving Your Cell-Based Research with Real-Time Assays

Cell-Based Assays

Tuesday, November 14, 2017

Terry Riss, PhD

With traditional cell-based endpoint assays you can miss important cellular events if measurement isn't taken at the appropriate time, particularly when characterizing a compound's effect on cells. Additionally, assay reagents can negatively affect cell health and endpoint assays can impede downstream multiplexing.

A Practical Guide to CRISPR-Mediated Gene Tagging with a Bioluminescent Peptide

Cell-Based Assays

Tuesday, October 24, 2017

Marie Schwinn, PhD

CRISPR/Cas9 gene editing has emerged as a powerful tool for making precise genomic modifications. Although often used to create gene knockouts, the technology also enables targeted knock-in of a specific sequence. An exciting application is the endogenous tagging of proteins expressed under native regulatory conditions. In this webinar, we will detail a simple and efficient cloning-free method for CRISPR-mediated endogenous gene tagging, focusing on knock-in of the sensitive, bioluminescent HiBiT tag.

Improved Chemistry for NGS Library Cleanup and Size Selection

Genomics

Tuesday, October 10, 2017

Charles Cowles, PhD

Current size-selective DNA purification methods need improvement with respect to sample loss, poor reproducibility, high viscosity and recovery of undesired high MW fragments. With new size selection chemistry, you can improve yield, selectivity, usability, and functionality in NGS library cleanup and size selection, as well as PCR cleanup and other applications.

Applications of a Bioluminescent Peptide Tag: Simple, Quantitative Protein Detection Down to Endogenous Levels

Cell-Based Assays

Tuesday, September 26, 2017

Christopher Eggers, PhD

Regulated protein abundance and surface expression are fundamental to normal cellular physiology and dysregulation underlies many disease states. Monitoring changes in protein abundance usually involves labor-intensive, antibody-based methods that yield only semi-quantitative results. This webinar will introduce a bioluminescent tagging system that can quantify proteins in minutes using simple, antibody-free methods. This system offers wide dynamic range and sensitivity compatible with detecting even endogenously expressed proteins when combined with CRISPR gene editing.

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