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Tuesday, November 14, 2017
Terry Riss, PhD
With traditional cell-based endpoint assays you can miss important cellular events if measurement isn't taken at the appropriate time, particularly when characterizing a compound's effect on cells. Additionally, assay reagents can negatively affect cell health and endpoint assays can impede downstream multiplexing.
Tuesday, October 24, 2017
Marie Schwinn, PhD
CRISPR/Cas9 gene editing has emerged as a powerful tool for making precise genomic modifications. Although often used to create gene knockouts, the technology also enables targeted knock-in of a specific sequence. An exciting application is the endogenous tagging of proteins expressed under native regulatory conditions. In this webinar, we will detail a simple and efficient cloning-free method for CRISPR-mediated endogenous gene tagging, focusing on knock-in of the sensitive, bioluminescent HiBiT tag.
Tuesday, October 10, 2017
Charles Cowles, PhD
Current size-selective DNA purification methods need improvement with respect to sample loss, poor reproducibility, high viscosity and recovery of undesired high MW fragments. With new size selection chemistry, you can improve yield, selectivity, usability, and functionality in NGS library cleanup and size selection, as well as PCR cleanup and other applications.
Tuesday, September 26, 2017
Christopher Eggers, PhD
Regulated protein abundance and surface expression are fundamental to normal cellular physiology and dysregulation underlies many disease states. Monitoring changes in protein abundance usually involves labor-intensive, antibody-based methods that yield only semi-quantitative results. This webinar will introduce a bioluminescent tagging system that can quantify proteins in minutes using simple, antibody-free methods. This system offers wide dynamic range and sensitivity compatible with detecting even endogenously expressed proteins when combined with CRISPR gene editing.
Tuesday, September 19, 2017
Ulrike Herbrand, PhD
In past years, the requirement for bioactivity testing changed from later phase lot release and stability testing to additional applications like biocomparability testing for follow-on biologics, accelerated stress condition testing and confirmation of successful production scale up. Therefore a suitable bioassay is needed at a very early time point. This webinar will look at a wide variety of therapeutic proteins and illustrate some best practices in method development that results in reliable, reproducible and precise assays, and discuss which assays may be best for your product and development program.