Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Making a Long Story Short: Quick Bacterial Diagnostics with High-Molecular Weight DNA

In this webinar, you will learn about:

  • The importance of HMW DNA in pathogen genomics
  • Application of long-read sequencing in bacterial diagnostics
  • Implementation of genomics in innovative all-in-one bacterial diagnostics


Bacterial diagnostics in human and veterinary medicine relies majorly on bacterial cultures or targeted short amplicon PCR-based tests. While culture-based assays are time-consuming or restricted for various important species (up to 2 weeks for Mycoplasma spp.), molecular assays provide a quick output. Hence, combined approaches are required to deliver answers on species identification, typing & antimicrobial susceptibility testing (AST). Making these long diagnostic stories short has been facilitated by the release of new affordable and real-time sequencing approaches (e.g. Oxford Nanopore Technologies). Exploiting long-read sequencing to the most, requires the generation of High-Molecular Weight (HMW) nucleic acids.

Rapid MinION sequencing could be performed on various bacterial species using HMW DNA extracts from the Wizard® Genomic DNA purification kit (Promega). This allowed us to collect more comprehensive data in real-time for faster species identification, typing & verification of the presence of virulence factor genes and antimicrobial resistance mediators. In addition, implementation of longer bacterial reads (N50 = ±20kbp) required less sequencing time to obtain 100x covered complete & high-quality bacterial genomes from datasets of Gram-positive, Gram-negative & Mycoplasma spp. bacteria. 

In conclusion, longer DNA molecules allow to shorten a long diagnostics workflow by quickly generating complete & high-quality bacterial genomes in an all-in-one sequencing-based bacterial diagnostic tests in human and veterinary medicine.



Nick Vereecke
Research & Development Scientist
PathoSense BV

Nick Vereecke, a R&D Scientist at PathoSense BV (Belgium), is a passionate Baekeland supported PhD Fellow (Flanders Innovation & Entrepreneurship). At the new Ghent University spin-off, he performs state-of-the-art research on viral & bacterial diagnostics in veterinary medicine in collaboration with different laboratories at Ghent University. With a great interest for various applications of (long-read) sequencing, his aim is to revolutionize current identification, (virulence) typing & antimicrobial resistance of infectious diseases in veterinary (and human) medicine. 

Ruchika Sharma, PhD
Product Specialist - Genomic Solutions
Promega Corporation

Ruchika Sharma is a Product Specialist in the Genomic Solutions Group at Promega Corporation. She manages a portfolio of Promega’s sample preparation products for in-vitro diagnostics and manual purification and works with customers in different market segments globally to understand and address their unmet sample preparation needs. She also works with Promega’s cross-functional teams to provide solutions tailored to customer needs for any specific applications of Promega’s products, including the Wizard® HMW DNA Extraction Kit for isolation of high-molecular weight DNA for long read sequencing. Ruchika holds a Ph.D. in Biochemistry and Molecular Biology from the Indian Institute of Science and did her postdoctoral training at the National Institutes of Health and the University of Wisconsin-Madison, focusing on the role of molecular chaperones in protein folding and human diseases.