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Small Molecule Profiling and Assay Development

Our custom assay services can help to accelerate your small molecule drug discovery and development workflow. These comprehensive services are built around award-winning bioluminescent technology for sensitive, high-throughput and biologically relevant results.

Targeted Protein Degradation/CRISPR Knock-in

The combination of CRISPR/Cas9 knock-in and live-cell bioluminescent detection methods offers a novel approach to study endogenous biology. Changes in protein abundance can be monitored in real time to better understand cellular protein dynamics.

SERVICES INCLUDE

  • Custom knock-in cell line development; learn more about available cell lines
  • Protein degradation profiling with optional cell viability monitoring

NanoBRET™ Target Engagement Assays

The NanoBRET™ Target Engagement Assays are live-cell binding assays that use an energy transfer technique to measure molecular proximity. These assays also provide information about compound-target residence time and can be used to screen a panel of inhibitors to compare IC50 values.

SERVICES INCLUDE

  • Tracer synthesis (from total synthesis to dye conjugation of target molecules)
  • Assay development for novel protein targets
  • Compound profiling of existing targets (e.g., CRBN, VHL, RAS, PARP)
  • Cellular permeability
  • Thaw-and-Use (assay-ready) cells

Compound Profiling Services

Get more biologically relevant results from your early-stage drug discovery screening. Rely on proven, highly sensitive luminescent assay readouts for target identification, validation and lead optimization. Test your compounds against any Promega assay!

SERVICES INCLUDE

  • Selectivity profiling in live cells, using NanoBRET™ Target Engagement (TE) against 192 or 234 kinase proteins with dose-response follow-ups
  • Custom profiling for kinases not in the standard panels
  • Protein degradation profiling with optional cell viability monitoring
  • NanoBRET™ TE Assays–RAS, NLRP3, PARPs
  • Protein-protein interaction assays

Protein:Protein Interaction (PPI) Assays

The bright luminescence and small size of NanoLuc® Luciferase allows sensitive detection of protein interactions in live cells using BRET (NanoBRET™) or complementation reporter-based (NanoBiT®) methods. These assays enable detection of protein interactions at endogenous expression levels in high-throughput, multiwell plate formats.

SERVICES INCLUDE

  • NanoBRET™ PPI Assays: Vectors, assay optimization, stable cell line development, compound testing
  • NanoBiT® PPI Assays: Vectors, assay optimization, stable cell line development, compound testing

Lumit™ Technology for Immunoassays

Lumit™ Immunoassays provide a simple and fast alternative to conventional immunoassay methods, including sandwich ELISAs and Western blots. The bioluminescence-based method can be completed in as little as 30 minutes.

SERVICES INCLUDE

  • SmBiT® and LgBiT® antibody labeling for X-Y antibodies that can be used to determine the optimal Ab pair for an immunoassay
  • Bulk labeling of selected antibodies from 1mg to 10mg
  • Testing primary Ab pairs with our pre-labeled secondary Ab detection system
  • Custom immunoassay development

Reporter Gene Assays

Reporter gene assays can be used to investigate a variety of cellular processes using bioluminescent light output as a sensitive measure of biological activity. We offer a full range of assay products and tools for your research.

SERVICES INCLUDE

  • Reporter vectors
  • Assay optimization
  • Stable cell line development
  • Compound testing

Do you have questions? Our Small Molecule Drug Discovery team has answers!