Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Small Molecule Profiling and Assay Development

Our custom assay services can help to accelerate your small molecule drug discovery and development workflow. These comprehensive services are built around award-winning bioluminescent technology for sensitive, high-throughput and biologically relevant results.

Targeted Protein Degradation/CRISPR Knock-in

The combination of CRISPR/Cas9 knock-in and live-cell bioluminescent detection methods offers a novel approach to study endogenous biology. Changes in protein abundance can be monitored in real time to better understand cellular protein dynamics.


  • Custom knock-in cell line development; learn more about available cell lines
  • Protein degradation profiling with optional cell viability monitoring

NanoBRET™ Target Engagement Assays

The NanoBRET™ Target Engagement Assays are live-cell binding assays that use an energy transfer technique to measure molecular proximity. These assays also provide information about compound-target residence time and can be used to screen a panel of inhibitors to compare IC50 values.


  • Tracer synthesis (from total synthesis to dye conjugation of target molecules)
  • Assay development for novel protein targets
  • Compound profiling of existing targets (e.g., CRBN, VHL, RAS, PARP)
  • Cellular permeability
  • Thaw-and-Use (assay-ready) cells

Compound Profiling Services

Get more biologically relevant results from your early-stage drug discovery screening. Rely on proven, highly sensitive luminescent assay readouts for target identification, validation and lead optimization. Test your compounds against any Promega assay!


  • Selectivity profiling in live cells, using NanoBRET™ Target Engagement (TE) against 192 or 240 kinase proteins with dose-response follow-ups
  • Custom profiling for kinases not in the standard panels
  • Protein degradation profiling with optional cell viability monitoring
  • NanoBRET™ TE Assays–RAS, NLRP3, PARPs
  • Protein-protein interaction assays

Protein:Protein Interaction (PPI) Assays

The bright luminescence and small size of NanoLuc® Luciferase allows sensitive detection of protein interactions in live cells using BRET (NanoBRET™) or complementation reporter-based (NanoBiT®) methods. These assays enable detection of protein interactions at endogenous expression levels in high-throughput, multiwell plate formats.


  • NanoBRET™ PPI Assays: Vectors, assay optimization, stable cell line development, compound testing
  • NanoBiT® PPI Assays: Vectors, assay optimization, stable cell line development, compound testing

Lumit™ Technology for Immunoassays

Lumit™ Immunoassays provide a simple and fast alternative to conventional immunoassay methods, including sandwich ELISAs and Western blots. The bioluminescence-based method can be completed in as little as 30 minutes.


  • SmBiT® and LgBiT® antibody labeling for X-Y antibodies that can be used to determine the optimal Ab pair for an immunoassay
  • Bulk labeling of selected antibodies from 1mg to 10mg
  • Testing primary Ab pairs with our pre-labeled secondary Ab detection system
  • Custom immunoassay development

Reporter Gene Assays

Reporter gene assays can be used to investigate a variety of cellular processes using bioluminescent light output as a sensitive measure of biological activity. We offer a full range of assay products and tools for your research.


  • Reporter vectors
  • Assay optimization
  • Stable cell line development
  • Compound testing

Do you have questions? Our Small Molecule Drug Discovery team has answers!