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CRISPR Ready-to-Use Reporter Cell Lines

CRISPR-engineered cell lines with a bioluminescent tag fused to the target of interest provide a convenient and quantifiable method for studying endogenous protein abundance. Live-cell assays using these cell lines enable the study of protein dynamics in real time.

Promega continues to expand our collection of HiBiTNanoLuc®, and HaloTag® CRISPR knock-in cell pools and clones, including both N and C-terminal fusions to many popular drug targets. Knock-ins are performed in common workhorse cell lines and, in many cases, the cell background that is most relevant to the biology being studied. Let our experts take care of the cell engineering so you can focus on your research goals.

Pools and Clones Options

prebuilt2

Pre-built & Purchasable
Ships in 1-3 weeks.

development2

In Development
May take up to 3 months to ship.

customizable2

Custom
Tell us your research needs.

Cell Line Pools and Clones

We offer a large selection of pre-built and in-development CRISPR knock-in cell lines. Search the table below to find your target or cell background of interest. Cell lines in development are indicated by asterisks (*).

Search List

Target Tag Terminus Background Clone/Pool
ADAR1(p150) HiBiT N A549 C
APEX HiBiT C HEK293 LgBiT P
AR HiBiT C 22Rv1 C
AR HiBiT C LNCaP clone FCG C
AR(V7) HiBiT C 22Rv1 C
ARAF HiBiT N A549 C
ARID1A HiBiT N ES-2 P
ARID1A HiBiT C ES-2 P
ARID1B HiBiT N TOV-21G P
ARID1B HiBiT C TOV-21G P
AURKA HiBiT C MOLT-4 P
AURKA HiBiT C PC-3 P
BCL10 HiBiT C NCI-H1299 C
BPTF HiBiT N HEK293 P
BPTF HiBiT C HEK293 P
BRAF HiBiT N A-549 C/P
BRAFV600E HiBiT N A375 C
BRD2 HiBiT N HEK293 LgBiT C
BRD4 HiBiT N A-549 C
BRD4 HiBiT N HEK293 C
BRD4 HiBiT N HEK293 LgBiT C
BRD4 HiBiT N PC-3 P
BRD4 NanoLuc N HEK293 C
BRD4 HiBiT-HaloTag N HEK293 LgBiT C
BRD7 HiBiT C HEK293 LgBiT C
BRD7 HiBiT C HEK293 P
BRD7 HiBiT N HEK293 LgBiT C/P
BRD9 HiBiT C HEK293 LgBiT C/P
BRD9 HiBiT N HEK293 LgBiT C/P
BRPF1 HiBiT N HEK293 P
BRPF1 HiBiT C HEK293 P
BTK HiBiT C Ramos C
CBL-B HiBiT C Jurkat C/P
CCNE1 HiBiT C HEK293 C
CCNE1 HiBiT C NIH:OVCAR-3 C
CCNK HiBiT N A549 C
CDK1 HiBiT C HEK293 C
CDK2 NanoLuc C HEK293 C
CDK2 HiBiT C HEK293 C
CDK4 HiBiT C HEK293 P
CDK4 HiBiT N K-562 P
CDK4 NanoLuc N K-562 C
CDK4 NanoLuc N T-47D C
CDK6 HiBiT C K-562 P
CDK6 NanoLuc N K-562 C
CDK6 HiBiT C HEK293 C
CDK9 HiBiT N HEK293 C
CDK9 HiBiT C HEK293 P
CDK12 HiBiT C A549 C
CREBBP HiBiT C U-2 OS C/P
CREBBP HiBiT C H929 P
CREBBP HiBiT C HEK293 P
CREBBP HiBiT N HEK293 P
CSNK1A1 HiBiT C HEK293 LgBiT C/P
CTNNB1 HiBiT N HEK293 LgBiT C
CTNNB1 HaloTag-HiBiT C HEK293 LgBiT C
deltaN-TP63 HiBiT N BxPC-3 C
DGKA (alpha) HiBiT N Jurkat C
DGKB (beta) HiBiT N A204 C
DGKZ (zeta) HiBiT N Jurkat C
DPP9 HiBiT C HEK293 LgBiT C
EP300 HiBiT C H929 P
EP300 HiBiT C HEK293 P
EP300 HiBiT N HEK293 P
EP300 HiBiT C U-2 OS C/P
ERBB3 (HER3) NanoLuc C AU565 C
ESR1 (ER) HiBiT C MCF7 C
EZH2 HiBiT C HEK293 C
FANCM HiBiT C HEK293 LgBiT P
FOXA1 HiBiT N 22Rv1 C
GAPDH HiBiT C HEK293 C
GSPT1 HiBiT N HEK293 LgBiT C/P
HDAC6 HiBiT C HeLa LgBiT C
HMOX1 HiBiT N A-549 C
HRI (EIF2AK1) HiBiT C HEK293 C
IKZF1 (Ikaros) HiBiT C Jurkat LgBiT C/P
IKZF2 (Helios) HiBiT C Jurkat LgBiT P
IKZF2 (Helios) HiBiT N Jurkat LgBiT C/P
IKZF3 (Aiolos) HiBiT N MM.1S C/P
IL17RA HiBiT C HeLa P
IL2 (CD38 KO) NanoLuc C Jurkat C
IRAK1 HiBiT C NCI-H1299 C
IRAK1 NanoLuc N Jurkat C
IRAK1 NanoLuc N THP-1 C
IRAK3 NanoLuc N THP-1 C
IRAK4 HiBiT C HeLa C
IRAK4 HiBiT C K-562 LgBiT C
IRAK4 HiBiT C NCI-H1299 C
JAK1 NanoLuc C HEK293 C
JAK2 NanoLuc C TF-1 C
JAK2 (V617F) HiBiT C Hel92.1.7 C
JAK2 (V617F) NanoLuc C Hel92.1.7 C
KLK3 HiBiT C 22Rv1 C
KRas (G12C) HiBiT N MIA PaCa-2 C
KRas (G12D) HiBiT N AsPC-1 C
KRas (G12D) HiBiT N HEK293 C
KRas (G12D) HiBiT N SW1990 C
KRas (G12S) HiBiT N A-549 C
KRas (G12V) HiBiT N SW620 C
KRas (G13D) HiBiT N NCI-H647 C
KRas (WT) HiBiT N NCI-H1299 C
KRas (WT) HiBiT N HEK293 C
MAP3K14 HaloTag-HiBiT C HeLa C
MAP4K1 (HPK1) NanoLuc N Jurkat C
MAP4K1 (HPK1) HiBiT N Jurkat C/P
MAP4K1 (HPK1) HiBiT-HaloTag N Jurkat C
MAPK14 NanoLuc C HEK293 C
MAPT (2N4R) HiBiT C IMR-32 C
MAPT (Tau 3R) HiBiT N IMR-32 C
MARC_1 HaloTag-HiBiT C HEK293 C
MET HiBiT C A-549 C
MET HiBiT C HeLa C
Myc (cMyc) HiBiT C HEK293 LgBiT C
MYH3 HiBiT C RD C
NFE2L2 (NRF2) HiBiT N A-549 C/P
NFE2L2 (NRF2) HiBiT N NCI-H460 C
NRAS (WT) HiBiT N HEK293 C
NRAS (Q61R) HiBiT N SW1271 C
NTN4 (NETRIN-4) HiBiT C DLD-1 P
PARP1 HiBiT C HeLa P
PAX8 HiBiT N NIH:OVCAR-3 P
PIK3C3 HiBiT N HEK293 LgBiT C
PKMYT-1 HiBiT N HEK293 LgBiT P
PKMYT-1 HiBiT C HEK293 LgBiT P
PLK1 HiBiT C A549 P
POLQ HaloTag-HiBiT C DLD-1 P
POLQ NanoLuc C DLD-1 P
POLQ NanoLuc N DLD-1 P
PTPN1 HiBiT N Jurkat LgBiT C
PTPN2 HiBiT N Jurkat LgBiT C
PTPN2 (isoform 2) HiBiT C Jurkat LgBiT C
PTPN6 (SHP1) HiBiT C THP-1 C
RAF1 (cRAF) HiBiT C A-549 C/P
RAF1 (cRAF) HiBiT C Panc 08.13 C
RAF1 (cRAF) HiBiT C HS766T C
RIPK2 HiBiT C THP-1 C
RUBCN HiBiT N HEK293 C
RUBCN HiBiT C HEK293 LgBiT C
SALL4 HiBiT N SK-N-DZ C
SALL4 HiBiT C SK-N-DZ C
SMAD3 HiBiT N HeLa LgBiT C
SMAD3 HiBiT-HaloTag N THP-1 C
SMARCA2 HiBiT C HeLa C/P
SMARCA4 HiBiT C HeLa C/P
SMARCA4 HiBiT C HeLa LgBiT P
STAT3 HiBiT C HeLa LgBiT C
STAT3-beta HiBiT C HeLa LgBiT C
SUCLA-2 HiBiT C 22Rv1 C
α-Synuclein HiBiT C HeLa C
TEAD2 HiBiT N HEK293 C
TNFRSF1A HiBiT C HeLa P
TREM2 HiBiT N THP-1 C
TREM2 HiBiT C THP-1 C
TREM2 HaloTag-HiBiT C THP-1 C
TYROBP (DAP12) HiBiT C THP-1 C
TYROBP (DAP12) HaloTag-HiBiT C THP-1 C
USP14 HiBiT C HEK293 LgBiT C/P
USP14 HiBiT N HEK293 LgBiT P
WRN HiBiT C HEK293 C

Would you like an offline copy of this table?

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Ordering Options

Contact us to get started with the cell lines you need or to stay updated on our growing list of available knock-in cell lines.

Want to Do-It-Yourself? Take advantage of our resources to make sure you get the results you need.

View DIY Resources

Do you have questions about using CRISPR knock-ins to study endogenous biology?

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