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Inflammation Assay

Assays to measure markers of inflammation or inflammasome activation include measurement of caspase-1 activity. The Caspase-Glo® 1 Assay provides a homogeneous, bioluminescent method to selectively measure caspase-1 activity, an essential component of the inflammasome, directly from cells or medium.

Standard methods such as Western blot analysis for detecting inflammasome activation are labor-intensive and time-consuming. They also do not measure the activity of caspase-1. The Caspase-Glo® 1 Inflammasome Assay offers a better alternative that provides easier, direct detection of caspase-1 activity in multiwell plates. The assay protocol is a simple, one-step addition that generates a stable, sensitive luminescent signal. This makes the assay ideal for multiplexing with other assays and for automated high-throughput screening applications.

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Inflammation Assay Basics

As more attention is focused on the role of inflammation in human disease, the ability to measure inflammasome activation is increasingly valuable. The role of inflammation in innate immunity protects us from disease; however, diseases caused by chronic inflammation are a growing medical challenge.

Inflammasomes are protein complexes induced by a diverse set of inflammatory stimuli. Innate immune cells respond to pathogens and other danger signals with the formation of various inflammasomes and the activation of caspase-1. Caspase-1 activation leads to processing and release of cytokines IL-1β and IL-18, and an immunogenic form of programmed cell death called pyroptosis.

Typically, inflammasome activation is monitored with Western blotting of processed caspase-1 or indirectly via determination of IL-1β or IL-18 processing or release, using Western blots or ELISAs, respectively. The bioluminescent Caspase-Glo® 1 assay enables rapid direct measurement of an upstream signaling event in the process of inflammation. This new approach enables studies on the kinetics of inflammasome activation and inactivation and allows a more precise assessment of caspase-1 mediated pyroptosis. This provides a means for high-throughput screening of inflammasome modulators.