Choose language: English
Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.
Our customer and technical support experts are available by telephone, fax, email or live chat to help.
From managing your profile to international ordering and payment options, we're here to help.
Place an order, check price, availability, delivery or order status.
Answer technical questions or request a seminar.
Current Items 0
Add multiple products
View Your Account and Past Online Orders
Customize Your Promega Experience
Create an Account
Catalog number selected:
Your price: Log In
This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
Click here to learn more about the Helix Collection »
Already a Helix Customer?
The assay provides a proluminescent caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a reagent optimized for caspase-3/7 activity, luciferase activity and cell lysis. Adding the single Caspase-Glo® 3/7 Reagent in an "add-mix-measure" format results in cell lysis, followed by caspase cleavage of the substrate. This liberates free aminoluciferin, which is consumed by the luciferase, generating a "glow-type" luminescent signal that is proportional to caspase-3/7 activity.
The stabilized luciferase and proprietary buffer system improve assay performance across a wide range of assay conditions, and the assay is less likely to be affected by compound interference unlike fluorescent- or colorimetric-based assays. The Caspase-Glo® 3/7 Assay is designed for use with multiwell plate formats using either purified enzyme or cells in culture and can be multiplexed with other cell-based assays. It also delivers excellent Z'-factor values in cell and purified enzyme models.
Jurkat cells were treated with anti-Fas mAb for 4.5 hours to induce apoptosis or were left untreated. Caspase-Glo® 3/7 Reagent was added directly to the cells in 96-well plates and incubated for 1 hour before recording luminescence. Each point represents the average of 4 wells. The "no cell" blank control value has been subtracted from each.
Using 100μl of Caspase-Glo® Reagent per assay in a 96-well format, Cat.# G8090 provides sufficient reagents to perform 25 assays; Cat.# G8091, 100 assays; Cat.# G8092 and G8093, 1,000 assays. Using 25μl of Caspase-Glo® Reagent per assay in a 384-well format, Cat.# G8090 provides sufficient reagents to perform 100 assays; Cat.# G8091, 400 assays; Cat.# G8092 and G8093, 4,000 assays.
Caspase-Glo® 3/7 Assay Technical Bulletin
(727 KB pdf)
Caspase-Glo® 3/7 Buffer
Caspase-Glo® 3/7 Substrate
PDF 0 B
Search for Specific Certificate:
U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.
U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.
Elongation factor 2 kinase promotes cell survival by inhibiting protein synthesis without inducing autophagy.
Measure caspase-2 activity with this homogeneous, luminescent assay.
Measure caspase-8 activity with this homogeneous, luminescent assay.
G8200, G8201, G8202
Measure caspase-9 activity with this homogeneous, luminescent assay.
G8210, G8211, G8212
Measure caspase-6 activity with this homogeneous, luminescent assay.
A complete and timely assay system for quantitative measurement of caspase-3 activity.
Sensitive fluorescent assay to measure active caspase-3 and -7.
G7792, G7790, G7791
A modified TUNEL Assay that provides accurate detection of apoptotic cells in situ.
Classic TUNEL Assay for the specific detection and quantitation of apoptotic cells.
A fluorescent pan-caspase inhibitor to dectect caspase activity in live cells.
High-performance multimode system for cell health and metabolism, reporter gene activity and NGS applications.
Let's find the product that meets your needs.