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Caspase-Glo® 3/7 3D Assay

Apoptosis Assay For 3D Cultures

  • Quantitative measurement of caspase 3/7 activity in 3D models
  • Simple add-mix-measure protocol
  • Scalable to 96- and 384-well plate formats

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Catalog number selected: G8981

$ 425.00
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Caspase-Glo® 3/7 3D Assay
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$ 425.00
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Highly Sensitive Caspase Assay for 3D Cultures

The Caspase-Glo® 3/7 3D Assay is a homogeneous, luminescent assay that measures caspase-3 and -7 activities present in apoptotic 3D cultures. The assay reagent contains a luminogenic caspase-3/7 substrate in cell lysis buffer. A one-step addition of the reagent results in cell lysis followed by caspase cleavage of the substrate and generation of a luminescent signal. Luminescence is proportional to the amount of caspase activity present.

The assay is validated for use in a wide range of 3D cell models and is suitable for high-throughput applications. It can also be multiplexed with other homogeneous assays so you can get more data from a single well.

Note: The Caspase-Glo® 3/7 3D Assay uses the same chemistry as the Caspase-Glo® 3/7 Assay with an improved protocol designed for use with 3D models.

Simple Add-Mix-Measure Protocol—No Sample Prep!

The Caspase-Glo® 3/7 3D Assay uses a simple add-mix-measure protocol without the need for washes or medium removal.

Caspase-Glo-3-7-3D-protocol

Compatible with Multiple 3D Models

The Caspase-Glo® 3/7 3D Assay is validated in various 3D models including spheroids with both tight and loose junctions generated by ultra-low attachment surface chemistries and Matrigel®-embedded micromasses.

HepG2

17213ma-w

HCT-116

17212ma-w

HCT-116 in Matrigel®

17214ma-w

Ideal for High-Throughput Screening

The Caspase-Glo® 3/7 3D Assay is designed for multiwell-plate formats up to 384-well, making it perfect for high-throughput screening of modulators of apoptosis in 3D cells (Z´ = 0.67). Unlike high-content imaging, the data is simple, quantitative and easy to interpret.

caspase-glo-3-7-3d-high-throughput-screening-z-prime

Multiplex with Cytotoxicity Assays in 3D Models

Get more data from a single sample well by performing two or three assays at once! For example, multiplexed results from 3D-compatible viability and cytotoxicity assays can provide a more complete characterization of an apoptotic response.
caspase-glo-3-7-3d-cytotoxicity-multiplex

Specifications

You are viewing: G8981 Change Configuration

What's in the box?

Item Part # Size

Caspase-Glo® 3/7 3D Buffer

G898A 1 × 10ml

Caspase-Glo® 3/7 3D Substrate

G899A 1 × 1 bottle

SDS

Choose language:

Download SDSPDF (289 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

Specifications

You are viewing: G8982 Change Configuration

What's in the box?

Item Part # Size

Caspase-Glo® 3/7 3D Buffer

G898B 1 × 100ml

Caspase-Glo® 3/7 3D Substrate

G899B 1 × 1 bottle

SDS

Choose language:

Download SDSPDF (289 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

Specifications

You are viewing: G8983 Change Configuration

What's in the box?

Item Part # Size

Caspase-Glo® 3/7 3D Buffer

G898A 10 × 10ml

Caspase-Glo® 3/7 3D Substrate

G899A 10 × 1 bottle

SDS

Choose language:

Download SDSPDF (289 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

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