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An Accurate Result for Every Well: Is Crosstalk Hurting Your Bioluminescent Assay Results?
RNA-Free Plant DNA Using the Maxwell® RSC PureFood GMO and Authentication Kit
Multiplexed Measurement of Protein Levels, Transcriptional Activation and Cell Viability in a Single Well using NanoDLR™ and CellTiter Fluor™ Assays on the GloMax® Discover System
Designing a Bioluminescent Reporter Assay: Normalization
Succinate-Glo™ JmjC Demethylase/Hydroxylase Assay
Rapid Digestion-Trypsin and -Trypsin/Lys-C Kits
When expressing your protein in a cell-free system, make sure the combination of promoter and DNA conformation is appropriate. For example, the T7 RNA polymerase promoter may express better in a linearized template while the SP6 promoter transcribes better in a circular DNA.
by Ed Himelblau