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HiBiT Protein Tagging System

Detect and quantify any tagged protein with a simple, luminescent signal.

HiBiT simplifies protein detection, providing a streamlined, alternative for detecting tagged proteins using a convenient, bioluminescence based method. With the dynamic range to detect proteins without overexpression, the convenience of a single-reagent-addition method, and live cell detection options, HiBiT technology opens up a world of possibilities for researchers studying protein biology. Compatibility with a sensitive and specific anti-HiBiT monoclonal antibody expands the detection options for HiBiT-tagged proteins, enabling traditional antibody-based methods for detection of the HiBiT tag.

How to tag proteins with HiBiT

Choose from 5 HiBiT Detection Methods

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LYTIC METHOD

Sensitive quantification of HiBiT-tagged proteins in cell lysates, IP complexes, or other cell-free systems.

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EXTRACELLULAR METHOD

Used with live cells to quantify surface-expressed or secreted proteins.

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BLOTTING SYSTEM

Detects any tagged protein on a blot in a few minutes with a simple luminescent signal.

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INTRACELLULAR LIVE-CELL DETECTION

Expresses LgBiT inside cells to quantify intracellular HiBiT-tagged proteins without requiring cell lysis.

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ANTIBODY-BASED DETECTION

Perform traditional antibody-based detection such as western blotting, immunocytochemistry, and pull-downs of HiBiT-tagged proteins.

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Example Applications of HiBiT Technology

Tag Endogenous Proteins via CRISPR Insertion—No Cloning Required

HiBiT technology makes CRISPR–mediated tag knock-ins accessible by eliminating the need for molecular cloning prior to insertion and simplifying detection of tagged proteins. The small tag size makes CRISPR insertion efficient and the bioluminescence enables antibody-free detection with the sensitivity to detect endogenous expression.

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Monitor Receptor Internalization

The HiBiT detection method eliminates the need for antibody-based methods for receptor internalization studies. With HiBiT technology, the multiple antibody binding steps and associated washes are eliminated from detection protocols. Simply add the detection reagent and measure a luminescent signal.

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Quantify Protein Abundance and Degradation

Classic epitope tagging methods are limited in throughput or sensitivity, require high-quality antibodies and may only yield semi-quantitative results. HiBiT tagging brings the simplicity and sensitivity of bioluminescence to studies on protein abundance, quantifying proteins down to endogenous levels, even those maintained at low expression levels.

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Speed and Simplify Western Blotting

The HiBiT blotting system is a sensitive and fast alternative to laborious Western blotting techniques that does not require an antibody.

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