HiBiT Protein Tagging System
Detect and quantify any tagged protein with a variety of sensitive methods.
The HiBiT epitope tag enables simple and flexible protein detection options. It provides a streamlined alternative for detecting epitope-tagged proteins using convenient, single-reagent-addition, bioluminescence-based methods. Compatibility with a sensitive and specific anti-HiBiT monoclonal antibody expands the utility of the HiBiT tag to include traditional antibody-based methods. All detection methods for the HiBiT tag have the dynamic range to detect proteins without overexpression, opening a world of possibilities for researchers studying protein biology.
HiBiT is a small, 11-amino-acid, epitope tag capable of producing a bioluminescent signal when bound to its complementation partner, LgBiT. HiBiT has unparalleled versatility and convenience with options for both simple and fast bioluminescent detection, and a sensitive and specific antibody for conventional detection methods.
Quantify Protein Abundance and Degradation
Classic epitope tagging methods are limited in throughput or sensitivity, require high-quality antibodies and may only yield semi-quantitative results. HiBiT tagging brings the simplicity and sensitivity of bioluminescence to studies on protein abundance, quantifying proteins down to endogenous levels, even those maintained at low expression levels.
Monitor Receptor Internalization
The HiBiT detection method eliminates the need for antibody-based methods for receptor internalization studies. With HiBiT technology, the multiple antibody binding steps and associated washes are eliminated from detection protocols. Simply add the detection reagent and measure a luminescent signal.
Speed and Simplify Western Blotting
The HiBiT blotting system is a sensitive and fast alternative to laborious Western blotting techniques that does not require an antibody.