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Lumit™ IL-1β Human/Mouse Immunoassay

No-Wash Immunoassay for IL-1β Detection in Cells

  • Fast add-and-read protocol performed directly on cells
  • Luminescent detection with just a standard plate-reading luminometer
  • Scalable to 96- or 384-well plates
  • Available as early access material; please contact us for more information.

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This product is discontinued
Lumit™ IL-1β Human/Mouse Immunoassay

Quantify Inflammasome Activation Without Tedious Wash Steps

The Lumit™ IL-1β Immunoassay quantitatively measures released IL-1β in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.

How the Lumit™ IL-1β Immunoassay Works

Primary antibodies to IL-1β were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-1β, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-1β levels.

Lumit IL-1beta assay mechanism of action

Simple One-Plate Protocol, No Wash Steps!

The simple, one-plate Lumit IL-1b assay protocol

Large Dynamic Range of Lumit™ Detection

The picomolar to nanomolar linear range of Lumit™ detection covers expected concentrations for most cell models.

Linear range of lumit IL-1b human immunoassay
Linear range of lumit IL-1b mouse immunoassay

Assay

IL-1β (Human) Immunoassay

IL-1β (Mouse) Immunoassay

Limit of Detection (LOD)

10pg/ml (3 SD above background)

8pg/ml (3 SD above background)

Dynamic Range

22pg/ml – 40ng/ml

11pg/ml – 20ng/ml

Minimal Detectable Dose (MDD)

7pg/ml (2 SD above background)

5pg/ml (2 SD above background)

Assay Time

70 minutes

Sample Type

Cell culture supernatants

SD=Standard Deviation

High-Throughput NLRP3 Inflammasome Inhibitor Screening

The add-and-read format of the Lumit™ IL-1β Immunoassay enables high-throughput screening for modulators of IL-1β release.
IL-1b inhibitor high throughput screening

The NLRP3 inflammasome inhibitor, MCC950, prevents caspase-1 activation and release of mature IL-1β. A titration of MCC950 was added to differentiated THP-1 cells (5 × 104/well) followed by LPS (20 EU/ml) or vehicle. The Lumit™ IL-1β (Human) Immunoassay was performed after 5 hours.

Coming Soon!

The following assays are in development and may be available as early access material or by custom order.

  • Lumit™ IL-2 (Human) Immunoassay
  • Lumit™ IL-6 (Human) Immunoassay
  • Lumit™ IFN-γ (Human) Immunoassay
  • Lumit™ TNF-α (Human) Immunoassay
  • Lumit™ IL-4 (Human) Immunoassay
  • Lumit™ IL-10 (Human) Immunoassay

Contact us for a current list of available kits and ordering information.

Email Us

Let's find the product that meets your needs.

Talk to a Scientist

Petra

Petra

Germany