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Lumit™ IL-1β Human/Mouse Immunoassay

No-Wash Immunoassay for IL-1β Detection in Cells

  • Fast add-and-read protocol performed directly on cells
  • Luminescent detection with just a standard plate-reading luminometer
  • Scalable to 96- or 384-well plates

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Catalog number selected: W6010

$ 530.00
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Lumit™ IL-1β Human/Mouse Immunoassay
Human/100 assays
$ 530.00
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Quantify Inflammasome Activation Without Tedious Wash Steps

The Lumit™ IL-1β Immunoassay quantitatively measures released IL-1β in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.

How the Lumit™ IL-1β Immunoassay Works

Primary antibodies to IL-1β were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-1β, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-1β levels.
16522ma

Simple One-Plate Protocol, No Wash Steps!

16545ma

Large Dynamic Range of Lumit™ Detection

The picomolar to nanomolar linear range of Lumit™ detection covers expected concentrations for most cell models.

Linear range of lumit IL-1b human immunoassay
Linear range of lumit IL-1b mouse immunoassay

Assay

IL-1β (Human) Immunoassay

IL-1β (Mouse) Immunoassay

Limit of Detection (LOD)

10pg/ml (3 SD above background)

8pg/ml (3 SD above background)

Dynamic Range

22pg/ml – 40ng/ml

11pg/ml – 20ng/ml

Minimal Detectable Dose (MDD)

7pg/ml (2 SD above background)

5pg/ml (2 SD above background)

Assay Time

70 minutes

Sample Type

Cell culture supernatants

SD=Standard Deviation

High-Throughput NLRP3 Inflammasome Inhibitor Screening

The add-and-read format of the Lumit™ IL-1β Immunoassay enables high-throughput screening for modulators of IL-1β release.
IL-1b inhibitor high throughput screening

The NLRP3 inflammasome inhibitor, MCC950, prevents caspase-1 activation and release of mature IL-1β. A titration of MCC950 was added to differentiated THP-1 cells (5 × 104/well) followed by LPS (20 EU/ml) or vehicle. The Lumit™ IL-1β (Human) Immunoassay was performed after 5 hours.

Coming Soon!

The following assays are in development and may be available as early access material or by custom order.

  • Lumit™ IL-2 (Human) Immunoassay
  • Lumit™ IL-6 (Human) Immunoassay
  • Lumit™ IFN-γ (Human) Immunoassay
  • Lumit™ TNF-α (Human) Immunoassay
  • Lumit™ IL-4 (Human) Immunoassay
  • Lumit™ IL-10 (Human) Immunoassay

Contact us for a current list of available kits and ordering information.

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Specifications

You are viewing: W6010 Change Configuration

What's in the box?

Item Part # SizeConcentration

Lumit™ Detection Substrate B

VB405A 1 × 160μl

Lumit™ Detection Buffer B

VB406A 1 × 3.2ml

Human IL-1β Standard

W116A 1 × 25μl10μg/ml

Anti hIL-1β mAb-SmBiT, 1000X

W117A 1 × 15μl

Anti hIL-1β mAb-LgBiT, 1000X

W118A 1 × 15μl

SDS

Choose language:

Download SDSPDF (424 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

Specifications

You are viewing: W6011 Change Configuration

What's in the box?

Item Part # SizeConcentration

Lumit™ Detection Substrate B

VB405B 1 × 1.25ml

Lumit™ Detection Buffer B

VB406B 1 × 25ml

Human IL-1β Standard

W116A 1 × 25μl10μg/ml

Anti hIL-1β mAb-SmBiT, 1000X

W117B 1 × 150μl

Anti hIL-1β mAb-LgBiT, 1000X

W118B 1 × 150μl

SDS

Choose language:

Download SDSPDF (424 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

Specifications

You are viewing: W6012 Change Configuration

What's in the box?

Item Part # SizeConcentration

Lumit™ Detection Substrate B

VB405A 5 × 160μl

Lumit™ Detection Buffer B

VB406A 5 × 3.2ml

Human IL-1β Standard

W116A 1 × 25μl10μg/ml

Anti hIL-1β mAb-SmBiT, 1000X

W117A 5 × 15μl

Anti hIL-1β mAb-LgBiT, 1000X

W118A 5 × 15μl

SDS

Choose language:

Download SDSPDF (425 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

Specifications

You are viewing: W7010 Change Configuration

What's in the box?

Item Part # SizeConcentration

Lumit™ Detection Substrate B

VB405A 1 × 160μl

Lumit™ Detection Buffer B

VB406A 1 × 3.2ml

Mouse IL-1β Standard

W119A 1 × 25μl10μg/ml

Anti mIL-1β mAb-SmBiT, 1000X

W120A 1 × 15μl

Anti mIL-1β mAb-LgBiT, 1000X

W121A 1 × 15μl

SDS

Choose language:

Download SDSPDF (424 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

Specifications

You are viewing: W7011 Change Configuration

What's in the box?

Item Part # SizeConcentration

Lumit™ Detection Substrate B

VB405B 1 × 1.25ml

Lumit™ Detection Buffer B

VB406B 1 × 25ml

Mouse IL-1β Standard

W119A 1 × 25μl10μg/ml

Anti mIL-1β mAb-SmBiT, 1000X

W120B 1 × 150μl

Anti mIL-1β mAb-LgBiT, 1000X

W121B 1 × 150μl

SDS

Choose language:

Download SDSPDF (424 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

Specifications

You are viewing: W7012 Change Configuration

What's in the box?

Item Part # SizeConcentration

Lumit™ Detection Substrate B

VB405A 5 × 160μl

Lumit™ Detection Buffer B

VB406A 5 × 3.2ml

Mouse IL-1β Standard

W119A 1 × 25μl10μg/ml

Anti mIL-1β mAb-SmBiT, 1000X

W120A 5 × 15μl

Anti mIL-1β mAb-LgBiT, 1000X

W121A 5 × 15μl

SDS

Choose language:

Download SDSPDF (424 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

Patents and Disclaimers

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

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