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Lumit™ TNF-α (Human) Immunoassay

No-Wash Immunoassay for TNF-α Detection

  • Fast add-and-read protocol performed directly on cells
  • Luminescent detection with just a plate-reading luminometer
  • Scalable to 96- or 384-well plates
  • Available as early access material; please contact us for more information

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This product is discontinued
Lumit™ TNF-α (Human) Immunoassay

Quantify Cytokine Release Without Tedious Wash Steps

The Lumit™ TNF-α Immunoassay quantitatively measures released TNF-α in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.

How the Lumit™ TNF-α Immunoassay Works

Primary antibodies to TNF-α were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of TNF-α, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to TNF-α levels.

How the Lumit™ IL-2 Immunoassay Works

Simple One-Plate Protocol, No Wash Steps

Lumit™ IL-2 Immunoassay Workflow

Large Dynamic Range of Lumit™ TNF-α Detection

The pg/ml to ng/ml linear range of Lumit™ detection covers expected concentrations for most cell models.

lumit tnf-alpha standard curve 17850ma

Assay

TNF-α Human Immunoassay

Limit of Detection (LOD)

3pg/ml (3 SD above background)

Dynamic Range

18.2pg/ml – 25ng/ml

Assay Time

70 minutes

Sample Type

Cell culture supernatants

SD=Standard Deviation

Sensitive Detection of TNF-α Without Sample Dilution

Data showing sensitive detection of TNF-α without sample dilution

When treated with a Cell Stimulation Cocktail (CSC), human PBMCs release TNF-α. After treatment for 24, 48 or 72 hours, Lumit™ TNF-α Human Immunoassay reagents were added directly to cell wells to measure TNF-α release. No sample dilution was required to measure quantities of TNF- α release.

Kit Contents

  • Recombinant Human TNF-α
  • Anti-hTNF-α mAb-LgBiT
  • Anti-hTNF-α mAb-SmBiT
  • Lumit™ Detection Substrate B
  • Lumit™ Detection Buffer B

For In Vitro Research Use Only.

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.


Explore Additional Cytokine Detection Assays: We offer a growing portfolio of assays to detect cytokines in cell culture samples. Can’t find your target in the list? Please enquire about early access to new assays in development.

Let's find the product that meets your needs.

Talk to a Scientist

Alessandro

Alessandro

Italy