Lumit™ Cytokine Immunoassays for Biologics
Activating immune cells is the mechanism of action for many biologics, such as monoclonal antibodies and cell and gene therapy products. Potency assays that measure cytokine release from immune cells are important for lot release and characterization purposes. Traditionally, ELISAs have been used for cytokine detection, but the process is often tedious and slow. Learn how Lumit™ Cytokine Immunoassays can help you get results faster.
Lumit™ Cytokine Immunoassays can detect released cytokines directly in-well with cultured cells. With zero wash steps, you can get results in 70 minutes or less!
For a biotech that understands time is of significant value, the Lumit™ Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.
Jonathan Chow, Principal Scientist, Corner Therapeutics
The Lumit™ IL-6 (Human) Immunoassay (left) and standard ELISA (right) were used to quantify IL-6 release from PBMCs. The results are similar, while the Lumit™ Assay was completed in one fifth the time of the ELISA, with no transfer, wash steps or plate washer needed.
Lumit™ Cytokine Immunoassays are suitable for use as bispecific T cell engager potency assays, such as in the example IL-2 and IFN-γ release data below. See more data in this poster: Novel Bioluminescent Bioassays for the Discovery and Development of Molecular and Cellular T -Cell Redirecting Cancer Therapy
Purified CD8+ T cells (effector cells) were combined with Raji B cells (target cells) and a serial dilution of Blincyto® (CD3/CD19 bispecific T cell engager). IL-2 and IFN-γ were detected with the corresponding Lumit™ Cytokine Immunoassays to detect cytokine release from the effector cells.
Large Dynamic Range of Lumit™ Cytokine Immunoassays
The wide linear range of Lumit™ detection covers expected concentrations for most cell models. The performance characteristics of various Lumit™ Cytokine Immunoassays are listed below.
|Immunoassay||Lumit™ IL-2 (Human)||Lumit™ IL-4 (Human)||Lumit™ IL-6 (Human)||Lumit™ IL-10 (Human)||Lumit™ IFN-γ (Human)||Lumit™ TNF-α (Human)|
|Limit of Detection (LOD; 3 SD above background)||11.2pg/ml||6.7pg/ml||7.5pg/ml||7.4pg/ml||1.7pg/ml||2.9pg/ml|
|Minimal Detectable Dose (MDD; 2 SD above background)||7.2pg/ml||4.5pg/ml||5.0pg/ml||4.9pg/ml||1.1pg/ml||2.0pg/ml|
Each Lumit™ Cytokine Immunoassay Kit contains the following:
- Recombinant human cytokine standard
- Anti-cytokine mAb-SmBiT
- Anti-cytokine mAb-LgBiT
- Lumit™ Detection Substrate B
- Lumit™ Detection Buffer B
Lumit™ Immunoassay Labeling Kit and Detection Reagents
Antibody labeling kit and detection reagents to help develop Lumit™ Immunoassays.
VB2500, VB2010, VB2020, VB2030, VB4050, VB4060
Lumit™ HMGB1 Human/Mouse Immunoassay
HMGB1 quantitation with an add-mix-measure protocol completed in 90 minutes or less.
Lumit IL-1β Human/Mouse Immunoassay
Quantifies inflammasome activation by measuring released IL-1β using a simple, no-wash protocol.
W6010, W6011, W6012, W7010, W7011, W7012
- Lumit™ Cytokine Assays: Interpolating Data with the GloMax® Discover
- Blog Article: Understanding Inflammation: A Faster, Easier Way to Detect Cytokines in Cells
- Blog Article: The Cytokine Storm: Why Some COVID-19 Cases are More Severe
- Webinar: Lumit™ Immunoassays: An Easier Faster Method for Analyte Detection
- White Paper: Lumit™ Immunoassays: A Rapid and Sensitive Method for Analyte Detection
- Application Note: Comparison of Lumit™ and Cisbio HTRF® Assays for Measurement of Human TNF-α