Lumit® IFN-β (Human) Immunoassay
No-Wash Immunoassay for IFN-β
- Cell-based assay with fast add-and-read protocol
- Scalable and flexible assay format to meet your needs
- Luminescent detection with any microplate luminometer
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$ 597.00
Catalog Number: W1810
$ 3,887.00
Catalog Number: W1811
$ 2,234.00
Catalog Number: W1812
$ 135.00
Catalog Number: W149A-C
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Quantify IFN-β Release Without Tedious Wash Steps
IFN-β is an essential mediator of the innate immune response. It has potent antiviral properties, regulates the activity of various immune cells, and exhibits anti-inflammatory effects. IFN-β is used as a therapeutic agent to mitigate inflammation in diseases such as multiple sclerosis.
The Lumit® IFN-β Immunoassay quantitatively measures released IFN-β in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes. The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.
How the Lumit® IFN-β Immunoassay Works
Primary antibodies to IFN-β were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IFN-β, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IFN-β levels.
Simple One-Plate Protocol Requires No Wash Steps
Broad Linear Range of Lumit® IFN-β Detection
The picomolar to nanomolar linear detection range of the Lumit® IFN-β Immunoassay covers expected concentrations for most cell models.
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Specification |
Lumit® IFN-β (Human) Immunoassay |
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Dynamic Range |
18.2–25,000pg/ml |
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Limit of Detection (LOD) |
6.9pg/ml (3 SD above background) |
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Minimal Detectable Dose (MDD) |
4.6pg/ml (2 SD above background) |
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Assay Time |
70 minutes |
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Sample Type |
Cell culture supernatants |
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SD=Standard Deviation
For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.
Jonathan Chow, Principal Scientist, Corner Therapeutics
Featured Webinar
Optimizing the Lumit® IL-6 Immunoassay for High-Throughput Drug Screening
In this webinar, we share key considerations for scaling Lumit® Immunoassays from a single 96-well plate to many 364-well plates and offer tips for optimizing Lumit® Immunoassays for HTS applications. You will also hear Galapagos scientist Dr. Nick Verstraeten share how his team used the Lumit® IL-6 Immunoassay in a screening campaign for antiviral drugs.
Lumit® Detection of IFN-β Release from Immune Cells
THP-1 cells were plated in 96-well plate format at 50,000 cells/well in RPMI 1640 + 10% heat-inactivated FBS. Cells were treated in triplicate for 40 hours with increasing concentrations of 2′3′-cGAMP. LPS at 1μg/ml was added to some samples for the last 20 hours of treatment. Following treatment, IFN-β release was quantitated using the Lumit® IFN-β Immunoassay.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
Lumit® Detection Substrate B |
VB405A | 1 × 160μl |
Lumit® Detection Buffer B |
VB406A | 1 × 3.2ml |
Anti-hIFN-β mAb-SmBiT, 1000X |
W180A | 1 × 15μl |
Anti-hIFN-β mAb-LgBiT, 1000X |
W181A | 1 × 15μl |
Human IFN-β Standard |
W149A | 1 × 25μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Lumit® Detection Substrate B |
VB405B | 1 × 1.25ml |
Lumit® Detection Buffer B |
VB406B | 1 × 25ml |
Anti-hIFN-β mAb-SmBiT, 1000X |
W180B | 1 × 150μl |
Anti-hIFN-β mAb-LgBiT, 1000X |
W181B | 1 × 150μl |
Human IFN-β Standard |
W149A | 1 × 25μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Lumit® Detection Substrate B |
VB405A | 5 × 160μl |
Lumit® Detection Buffer B |
VB406A | 5 × 3.2ml |
Anti-hIFN-β mAb-SmBiT, 1000X |
W180A | 5 × 15μl |
Anti-hIFN-β mAb-LgBiT, 1000X |
W181A | 5 × 15μl |
Human IFN-β Standard |
W149A | 1 × 25μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Human IFN-β Standard |
W149A | 1 × 25μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Posters
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