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Lumit™ IL-4 (Human) Immunoassay

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No-Wash Immunoassay for IL-4 Detection

  • Fast add-and-read protocol performed directly on cells
  • Luminescent detection with just a plate-reading luminometer
  • Scalable to 96- or 384-well plates

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Catalog number selected: W6060

$ 569.00
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Lumit™ IL-4 (Human) Immunoassay
100 assays
$ 569.00
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Quantify Cytokine Release Without Tedious Wash Steps

The Lumit™ IL-4 Immunoassay quantitatively measures released IL-4 in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.

How the Lumit™ IL-4 Immunoassay Works

Primary antibodies to IL-4 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-4, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-4 levels.

How the Lumit™ IL-2 Immunoassay Works

Simple One-Plate Protocol, No Wash Steps

Lumit™ IL-2 Immunoassay Workflow

Large Dynamic Range of Lumit™ IL-4 Detection

The pg/ml to ng/ml linear range of Lumit™ detection covers expected concentrations for most cell models.

lumit il-4 standard curve 17845ma

Assay

IL-4 Human Immunoassay

Limit of Detection (LOD)

6.7pg/ml (3 SD above background)

Dynamic Range

18.2pg/ml – 25ng/ml

Minimal Detectable Dose (MDD)

4.5pg/ml (2 SD above background)

Assay Time

70 minutes

Sample Type

Cell culture supernatants

SD=Standard Deviation

Sensitive Quantitation of Low-Level IL-4 Release

Quantitation of low-level IL-4 release with the lumit IL4 assay.

When treated with a Cell Stimulation Cocktail (CSC), T helper type 2 (Th2) cells and human PBMCs (PBMC) each release low levels of IL-4. Following 24 hours of CSC treatment, Lumit™ IL-4 Immunoassay reagents were added directly to cell wells to measure IL-4 release.

Same-Well Multiplexing with Viability and Cytotoxicity Assays

Same-well multiplexing with viability and cytotoxicity assays.18097ma

Human T helper type 2 (Th2) cells were plated in 96-well plate format, primed overnight with hIL-2 and treated with increasing concentration of cell stimulation cocktail (CSC) for 24 hours. Following treatment, CellTox™ Green Cytotoxicity Assay and the Lumit™ IL-4 (Human) Immunoassay reagents were added to determine cytotoxicity and IL-4 release. CellTiter-Glo® Cell Viability Assay reagent was added to determine cell viability. A dose-dependent increase in IL-4 was observed, with some degree of increased cytotoxicity and decreased cell viability.

Explore Additional Cytokine Detection Assays

We offer a growing portfolio of assays to detect cytokines in cell culture samples.

Can’t find your target in the list?

Please enquire about early access to new assays in development.

For a biotech that understands time is of significant value, the Lumit™ Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.

Jonathan Chow, Principal Scientist, Corner Therapeutics

Specifications

You are viewing: W6060 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit™ Detection Substrate B

VB405A 1 × 160μl

Lumit™ Detection Buffer B

VB406A 1 × 3.2ml

Human IL-4 Standard

W125A 1 × 25μl 10μg/ml

Anti-hIL-4 mAb-SmBiT, 500X

W126A 1 × 30μl

Anti-hIL-4 mAb-LgBiT, 500X

W127A 1 × 30μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W6062 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit™ Detection Substrate B

VB405A 5 × 160μl

Lumit™ Detection Buffer B

VB406A 5 × 3.2ml

Human IL-4 Standard

W125A 1 × 25μl 10μg/ml

Anti-hIL-4 mAb-SmBiT, 500X

W126A 5 × 30μl

Anti-hIL-4 mAb-LgBiT, 500X

W127A 5 × 30μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Specifications

You are viewing: W6061 Change Configuration

What's in the box?

Item Part # Size Concentration

Lumit™ Detection Substrate B

VB405B 1 × 1.25ml

Lumit™ Detection Buffer B

VB406B 1 × 25ml

Human IL-4 Standard

W125A 1 × 25μl 10μg/ml

Anti-hIL-4 mAb-SmBiT, 500X

W126B 1 × 300μl

Anti-hIL-4 mAb-LgBiT, 500X

W127B 1 × 300μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

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