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Lumit™ HMGB1 Human/Mouse Immunoassay

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No-Wash Immunoassay for HMGB1 Detection

  • Fast add-and-read assay performed directly on cells
  • Detects human or mouse HMGB1
  • Luminescent detection with just a plate-reading luminometer
  • Available as early access material; please contact us for more information

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This product is discontinued
Lumit™ HMGB1 Human/Mouse Immunoassay

Fast, No-Wash Detection of HMGB1

High-Mobility Group Box 1 (HMGB1) functions as a damage-associated molecular pattern (DAMP) released from cells in inflammatory responses. HMGB1 is often measured as a key biomarker of immunogenic cell death, a specialized form of cell death that results in an immune response. HMGB1 also functions as a proinflammatory cytokine. Current methods to detect HMGB1, such as ELISA, require tedious wash steps and lengthy protocols. Lumit™ technology simplifies HMGB1 quantitation with an add-mix-measure protocol completed in 90 minutes or less.

How the Lumit™ HMGB1 Immunoassay Works

Primary antibodies to HMGB1, selected for their specific and sensitive detection, are labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of HMGB1, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescence signal proportional to HMGB1 levels. The assay detects both human and mouse HMGB1.

How the lumit HMGB1 assay works.

Simple One-Plate Protocol, No Wash Steps

lumit HMGB1 immunoassy workflow

Large Dynamic Range of HMGB1 Detection

Due to the homology of human and mouse HMGB1, the kit can be used for both species with minimal performance differences.

Data showing dynamic range of HMGB1 detection with the Lumit HMGB1 assay.

Target

Human HMGB1

Mouse HMGB1

Limit of Detection 
(3SD above background)

1ng/ml 

3ng/ml 

Dynamic Range

1–729ng/ml

3–2187ng/ml

Assay Time

90 minutes

Sample Type

Cell culture samples

SD=Standard Deviation

Detect Drug-Induced Immunogenic Cell Death in Various Cell Culture Models

Drug-induced immunogenic cell death detected with HMGB1 assay.

Human U2OS cells (left) and mouse EL4 cells (right) were treated with doxorubicin for 24 hours. After 24 hours, the Lumit™ HMGB1 Immunoassay was performed directly on cell culture samples to measure HMGB1 released from treated cells.

Kit Contents

  • Recombinant human HMGB1
  • Anti-hHMGB1-mAb LgBiT
  • Anti-hHMGB1-mAb SmBiT
  • Lumit™ Detection Substrate B
  • Lumit™ Detection Buffer B

Kits available for 100 assays (1 × 96-well plate) or 1,000 assays (10 × 96-well plates).

For Research Use Only. Not for Use in Diagnostic Procedures.

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.


Also Available: Live-Cell Kinetic Measurement of Extracellular ATP

The RealTime-Glo™ Extracellular ATP Assay kinetically measures the release of ATP from dying cells so you can assess immunogenic cell death induction in real time. Use this assay to complement the Lumit™ HMGB1 Immunoassay for a more complete picture of immunogenic cell death.

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