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Protein analysis

Live-Cell Protein Interactions

Protein:protein interactions (PPIs) are essential elements of cellular signal transduction networks. Although numerous approaches exist to monitor PPIs in vitro, methods for intracellular detection have been more limited.

The bright luminescence and small size of NanoLuc® Luciferase allows sensitive detection of protein interactions in vivo using BRET (NanoBRET™) or complementation reporter-based (NanoBiT®) methods.

The NanoBRET™ and NanoBiT® technologies featured here enable detection of protein interactions at endogenous expression levels in ways that have not been possible before. The reversible assay technologies allow you to study both induction and inhibition of protein interactions using either NanoBRET™ or NanoBiT® methods in live-cell assay formats that allow for interaction kinetics to be monitored in real time. Both technologies are compatible with bench scale or high-throughput, multiwell plate methods.

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What are Live-Cell Protein Interactions?

Traditional methods for studying interactions between proteins, like co-immunoprecipitation, do not provide data in the context of the cellular environment. 

Using Bioluminescence Resonance Energy Transfer (BRET), it is possible to quantitatively measure the interaction between proteins in live cells. In some cases, it is even possible to measure these interactions in real time, observing not only the formation of the interaction but also the dissociation of the partners.

BRET measures protein interactions using a bioluminescent donor fused to a protein of interest and a fluorescent receptor fused to its binding partner. The bioluminescent donor, usually a luciferase, does not excite the fluorophore using light, but transfers resonance energy through dipole-dipole coupling. To transfer resonance energy, the donor must be within 10nm of the receptor and in the proper orientation, making the technique useful for measuring proteins in close proximity. The spectral overlap between the bioluminescent donor and fluorescent acceptor is an important factor for achieving optimal energy transfer while minimizing background signal, and proper instrumentation is required to separate the donor and acceptor signals.

Protein interactions can also be detected in live cells using complementation-based assays, where an active reporter enzyme is generated when subunit components are brought into proximity by two interacting protein partners. Complementation-based assays typically provide a direct measure of reporter activity with simpler instrumentation requirements.

For protein interaction studies in live cells, it is important to ensure that the technology used does not significantly interfere with normal protein function, and to ensure that the enzyme subunits do not self-associate in the absence of the interacting protein partners. The small size and bright signal of Nanoluc® luciferase accommodates detection at low, native expression levels with minimal interference with natural protein function. The low self-affinity of the NanoBiT® subunits combined with live-cell detection offers kinetic, real-time analysis of protein interaction dynamics in living cells.

We offer a complete portfolio of protein analysis products supported by a growing library of educational resources, including technology guides, on-demand webinars, scientific articles and protocols.

Use the links below to learn more about protein analysis products, or to explore learning resources covering topics such as in vitro protein expression, protein purification methods and live-cell protein interaction analysis.