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TnT® Quick Coupled Transcription/Translation System

Single-Tube, Coupled Transcription/Translation for Eukaryotic Cell-Free Protein Expression

  • Functional protein is expressed in one hour, ready for use without further purification
  • Use protein for protein:protein and protein:nucleic acid interactions, protein modification and more
  • Choice of SP6 or T7 promoter

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Catalog number selected: L1170

$ 640.00
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TnT® Quick Coupled Transcription/Translation System
T7/40 reactions
$ 640.00
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Express Protein from PCR Fragments or Plasmid DNA

The TnT® Quick Coupled Transcription/Translation Systems provide convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression. These in vitro translation systems combine the RNA Polymerase, nucleotides, salts, amino acids and Recombinant RNasin® Ribonuclease Inhibitor with the reticulocyte lysate solution to form a single TnT® Quick Master Mix.

The TnT® Quick Coupled Transcription/Translation System is available in two configurations for the expression of genes cloned downstream from either the T7 or SP6 RNA polymerase promoters. To use these cell-free protein expression systems, 0.2–2.0μg of circular plasmid DNA containing a T7 or SP6 promoter, or a PCR-generated fragment containing a T7 promoter, is added to an aliquot of the TnT® Quick Master Mix and incubated in a 50μl reaction volume for 60–90 minutes at 30°C. The expression reaction produces significant quantities of protein for a variety of applications including GST pull-downs and gel shift assays.

These mammalian-based systems express soluble, functional proteins that are post-translationally modified, unlike E. coli-based systems. Expressed proteins can be used directly after expression; no additional protein purification needed. 

90 Minute In Vitro Translation Protocol

Schematic showing DNA to protein expression in 60–90 minutes.

Applications of In Vitro Translation Systems

The TnT® Transcription/Translation Systems provide a simplified and accelerated method to perform in vitro protein translation. These systems have proven to be useful for many downstream applications including, but not limited to, glutathione-S-transferase (GST) pull-downs, gel shift assays, co-immunoprecipitation, characterization of protein modifications, protein:protein and protein:nucleic acid interactions and protein activity studies.

The animations and article below discuss a few of these applications in more detail. For more information about in vitro protein translation systems and how they can be used, see the Protein Expression chapter of the Protocols & Applications Guide.

GST Pull-Downs

Gel Shift Assays

Co-immunoprecipitation 

Characterization of Protein Modifications

Further Downstream: Expanded Applications                                                                                                                                    for TNT® Transcription/Translation Systems

The TnT® Transcription/Translation Systems are a convenient method for in vitro
protein translation. These systems are useful for many downstream applications,
including protein:protein interactions and protein:DNA interactions.

In this article, we review several publications that utilized TnT® Systems and
explore how these systems can be employed beyond these initial protein
translation applications.

Read More About Protein Translation Applications

Specifications

You are viewing: L1170 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Methionine

L118A 1 × 50μl1mM

T7 TnT® PCR Enhancer

L216A 1 × 100μl

Luciferase T7 Control DNA

L482A 1 × 5μg0.5μg/μl

Luciferase Assay Reagent

L486A 1 × 250μl

TnT® T7 Quick Master Mix

L520A 8 × 200μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product

SDS

Choose language:

Download SDSPDF (163 KB) – English (United States)

Certificate of Analysis

See all certificates for L1170

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L1171 Change Configuration

What's in the box?

Item Part # SizeConcentration

Methionine

L118A 1 × 50μl1mM

T7 TnT® PCR Enhancer

L216A 1 × 100μl

Luciferase T7 Control DNA

L482A 1 × 5μg0.5μg/μl

TnT® T7 Quick Master Mix

L520A 1 × 200μl

SDS

Choose language:

Download SDSPDF (115 KB) – English (United States)

Certificate of Analysis

See all certificates for L1171

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L2080 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Methionine

L118A 1 × 50μl1mM

TnT® SP6 Quick Master Mix

L209A 8 × 200μl

Luciferase SP6 Control DNA

L474A 1 × 5μg0.5μg/μl

Luciferase Assay Reagent

L486A 1 × 250μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product

SDS

Choose language:

Download SDSPDF (142 KB) – English (United States)

Certificate of Analysis

See all certificates for L2080

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L2081 Change Configuration

What's in the box?

Item Part # SizeConcentration

Methionine

L118A 1 × 50μl1mM

TnT® SP6 Quick Master Mix

L209A 1 × 200μl

Luciferase SP6 Control DNA

L474A 1 × 5μg0.5μg/μl

SDS

Choose language:

Download SDSPDF (94 KB) – English (United States)

Certificate of Analysis

See all certificates for L2081

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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