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Trypsin/Lys-C Mix, Mass Spec Grade

Improve Protein Digestion with Mix of Trypsin Gold and rLys-C

  • Standard overnight digestion, nondenaturing conditions
  • Increased peptide recovery, enhanced protein quantitation, improved reproducibility
  • Tolerant to trypsin-inhibiting contaminants

Size

Catalog number selected: V5071

$ 65.00
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This product is discontinued
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Trypsin/Lys-C Mix, Mass Spec Grade
20μg
$ 65.00
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Trypsin/Lys-C Mix, Mass Spec Grade: See What You've Been Missing

Using standard digestion conditions, Trypsin/Lys-C Mix improves peptide digestion over trypsin alone, increasing peptide recovery and resulting in better protein quantitation.
Trypsin digest illustration.

Increased Peptide and Protein Identifications Using a Standard Trypsin Digestion Protocol

Standard overnight trypsin digest schematic 11788ME-W-a
Trypsin LysC overnight digest schematic 11788ME-W-b
Data comparing trypsin and Trypsin/Lys-C digests.
Incomplete digestion with trypsin was evident after comparing peptide peak intensities in trypsin and Trypsin/Lys-C Mix digests.

Improved Reproducibility using Trypsin/Lys-C

Reproducible IgG (Rituximab) digestion  with Trypsin Lys-C mix.
Improved reproducibility of IgG (Rituximab) digestion when using the Trypsin/Lys-C Mix.

Tolerant To Trypsin-Inhibiting Contaminants

Trypsin LysC digestion results in fewer missed cleavages. 13058MC-W-a
Trypsin LysC increases protein identification compared to trypsin alone. 13058MC-W-b
Efficient digestion with Trypsin/Lys-C Mix in the presence of trypsin-inhibiting agents. Yeast protein extract was used as a model system.
Conclusions: Use of Trypsin/Lys-C resulted in fewer missed cleavages and a 20% increase in protein identifications.

Specifications

You are viewing: V5071 Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

V507A 1 × 20μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: V5072 Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

V507B 1 × 100μg

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: V5073 Change Configuration

What's in the box?

Item Part # Size

Resuspension Buffer

V181A 1 × 500μl

Trypsin/Lys-C Mix, Mass Spec Grade

V507A 5 × 20μg

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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