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NanoBRET™ Nano-Glo® Detection System

N1661_NanoBRET--Nano-Glo--Det-Sys-200-Assay_3

Dedicated Detection for NanoBRET™ Protein Interaction Assays

  • Measure protein interactions in live cells with full-length proteins
  • Study both on and off protein interactions with a reversible assay
  • Increased signal and lower background compared to conventional BRET assays

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Catalog number selected: N1661

$ 368.00
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NanoBRET™ Nano-Glo® Detection System
200 assays
$ 368.00
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32612632-L1-NanoBRET-PPI-Overview-Image

Detection Reagent for use with NanoBRET™ Protein Interaction Assays

The NanoBRET™ assay is a bioluminescence resonance energy transfer (BRET)-based assay that uses NanoLuc® Luciferase as the BRET energy donor and HaloTag® protein labeled with the HaloTag® NanoBRET™ 618 fluorescent Ligand as the energy acceptor to measure the interaction of two binding partners in live cells.

The NanoBRET™ Nano-Glo® Detection System provides the NanoBRET™ Nano-Glo® Substrate used by NanoLuc® Luciferase to generate the donor signal and the HaloTag® NanoBRET™ 618 Ligand for the fluorescent energy acceptor. The HaloTag® NanoBRET™ 618 Ligand is added directly to the cells during plating, and the NanoBRET™ Nano-Glo® Substrate is added to the sample just prior to measuring donor and acceptor emission.

For more details on using the NanoBRET™ assay for protein:protein interaction studies visit NanoBRET™ Technology for Protein Interactions. The NanoBRET™ Nano-Glo® Detection System is compatible with a diverse set of prebuilt or custom NanoBRET™ PPI Assays.

Note: Cat.# N1661 is sufficient for 200 assays in 96-well plates or 500 assays in 384-well plates.
Cat.# N1662 is sufficient for 1,000 assays in 96-well plates or 2,500 assays in 384-well plates.
Cat.# N1663 is sufficient for 10,000 assays in 96-well plates or 25,000 assays in 384-well plates.

NanoBRET™ PPI Assay Principle

Energy Transfer from NanoLuc to HaloTag NanoBRET Ligand
Depiction of energy transfer from a NanoLuc®-Protein A fusion (energy donor) to a fluorescently labeled HaloTag®-Protein B fusion (energy acceptor) upon interaction of Protein A and Protein B. 
Donor and Acceptor Signal Wavelengths for NanoBRET
Spectral separation of the NanoLuc® emission (460nm) and the fluorescent HaloTag® NanoBRET™ 618 Ligand emission (618nm), and calculation of the NanoBRET™ ratio.

Simple Protocol Saves Time

12750MA-W

Example Protein Interaction Assay Data

12751MA-W-a
Activation of the EGFR/GRB2 interaction by EGF induction.
12751MA-W-b

Inhibition of the BRD4/Histone H3.3 interaction by JQ1.

Specifications

You are viewing: N1661 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

HaloTag® NanoBRET™ 618 Ligand

G980A 1 × 20μl View Product

NanoBRET™ Nano-Glo® Substrate

N157A 1 × 50μl

SDS

Choose language:

Download SDSPDF (89 KB) – English (United States)

Certificate of Analysis

See all certificates for N1661

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(a) use NanoBRET™-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(b) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

U.S. Pat. No. 7,867,726 and other patents and patents pending.

U.S. Pat. Nos. 10,067,149 and 10,024,862 and other patents and patents pending.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730 and 9,951,373 and other patents and patents pending.

U.S. Pat. Nos. 9,056,885 and 10,139,400, European Pat. No. 2782916 and other patents pending.

Licensed under EP1295121 and EP1088233.

Specifications

You are viewing: N1662 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

HaloTag® NanoBRET™ 618 Ligand

G980A 5 × 20μl View Product

NanoBRET™ Nano-Glo® Substrate

N157A 5 × 50μl

SDS

Choose language:

Download SDSPDF (89 KB) – English (United States)

Certificate of Analysis

See all certificates for N1662

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(a) use NanoBRET™-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(b) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

U.S. Pat. No. 7,867,726 and other patents and patents pending.

U.S. Pat. Nos. 10,067,149 and 10,024,862 and other patents and patents pending.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730 and 9,951,373 and other patents and patents pending.

U.S. Pat. Nos. 9,056,885 and 10,139,400, European Pat. No. 2782916 and other patents pending.

Licensed under EP1295121 and EP1088233.

Specifications

You are viewing: N1663 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

HaloTag® NanoBRET™ 618 Ligand

G980B 1 × 1ml0.1mM View Product

NanoBRET™ Nano-Glo® Substrate

N157B 2 × 1.25ml View Product

SDS

Choose language:

Download SDSPDF (89 KB) – English (United States)

Certificate of Analysis

See all certificates for N1663

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(a) use NanoBRET™-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(b) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

U.S. Pat. No. 7,867,726 and other patents and patents pending.

U.S. Pat. Nos. 10,067,149 and 10,024,862 and other patents and patents pending.

U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730 and 9,951,373 and other patents and patents pending.

U.S. Pat. Nos. 9,056,885 and 10,139,400, European Pat. No. 2782916 and other patents pending.

Licensed under EP1295121 and EP1088233.

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