We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Glucose-Glo™ Assay

Detect Even Small Changes in Glucose

  • Measure glucose in a variety of sample types
  • No sample deproteinization required
  • Limit of glucose detection down to nM range
  • Glucose assay signal to background >1,000

Size

Catalog number selected: J6021

$ 375.00
Your price:
Add to Cart
This product is discontinued
Request a Sample
Not available in all countries
Add to Helix
Glucose-Glo™ Assay
5ml
$ 375.00
Your price: Log in
Change Configuration

Rapid, Selective and Sensitive Glucose Detection in Biological Samples

The Glucose-Glo™ Assay is a bioluminescent assay for rapid and sensitive measurement of glucose from a variety of sample types. The bioluminescent signal eliminates signal interference that colorimetric and fluorescent glucose assays suffer, and there is no need for deproteinization sample preparation steps. The Glucose-Glo™ Assay is suitable for detecting altered glucose consumption due to changes in glycolysis or glucose production during gluconeogenesis.

 

How the Assay Works

The Glucose-Glo™ Assay couples glucose oxidation and NADH production with a bioluminescent NADH detection system. Glucose dehydrogenase uses glucose and NAD+ to produce NADH. In the presence of NADH a pro-luciferin Reductase Substrate is converted by Reductase to luciferin that is then used by Ultra-Glo™ Recombinant Luciferase to produce light.

When Glucose Detection Reagent, which contains glucose dehydrogenase (GDH), NAD+, Reductase, Reductase Substrate and Luciferase, is added to a sample containing glucose at a 1:1 ratio, the enzyme-coupled reactions start and run simultaneously. The luminescent signal is proportional to the amount of glucose in the sample and increases until all glucose is consumed, at which time a stable luminescent signal is achieved.

Luminescent Signal is Proportional to Amount of Glucose Present

13887ma-w: Luminescence from glucose assay proportional to metabolite
Glucose titration curve. Serial dilutions of glucose were prepared and assay performed as described in TM494. 

Amenable to Your Throughput and Workflow Requirements

The Glucose-Glo™ Assay is a versatile detection kit that is amenable to high-throughput formats and compatible with many sample types including cells, lysates, tissue, plasma and serum. To simplify sample processing, the glucose detection kit uses processing methods that do not require sample centrifugation or spin columns. Simply dilute your samples, add inactivation and neutralization solutions (if needed), and assay all samples directly in 96- or 384-well plates.

 

Glucose Consumption by Adherent Lung Carcinoma A549 Cells

13888ma-w: Luminescent glucose assay measured consumption for 72 hours
A549 cells were plated at 5,000 (light blue bars) and 15,000 (dark blue bars) cells/well in medium. Wells with medium only were included as controls. At indicated time points, 2.5µl of medium was removed and diluted. Samples were stored as indicated before assaying as described in TM494. Red line represents luminescence values of medium-only controls.

Measure Glycolysis and Glutaminolysis from One Sample

Easily measure changes in cellular glycolysis and glutaminolysis by sampling medium over time. Direct measurement of four metabolites important to the energetic state of the cell—glucose, lactate, glutamate and glutamine—can be performed in parallel using the bioluminescent Glucose-Glo™, Lactate-Glo™, Glutamine/Glutamate-Glo™ and Glutamate-Glo™ Assays. No specialized instrument, plates or artificial medium is required. Sample processing compatible with all of the bioluminescent metabolite assays means the same sample can be used for detecting all four metabolites. This includes sample types such as culture medium, serum, plasma and tissue. The data below were produced by measuring metabolites using medium samples from the same cell cultures as described in Technical Manual TM494.

13836ma-w-a: Glucose consumption measured in metabolite multiplex assay.
13836ma-w-b: Lactate secretion measured in metabolite multiplex assay.
13836ma-w-c: Glutamine consumption measured in metabolite multiplex assay.
13836ma-w-d: Glutamate secretion measured in metabolite multiplex assay.

Advantages for Glucose-Glo™ Assay

Quickly Detect Glucose in a Variety of Sample Types: Measure in medium, cells, tissue and plasma. The glucose detection assay requires minimal preparation steps with no need for centrifugation and spin columns.

Detect Small Changes in Glucose Levels Across a Wide Range of Concentrations: Sensitive glucose assay with broad linearity better discriminates small changes in glucose compared to colorimetric and fluorometric glucose assays.

Gain More Information Per Sample: The glucose detection assay along with the other bioluminescent metabolite detection assays are amenable to measuring multiple metabolites from the same sample. Multiplex with cell viability assays for normalization.

Requires Only a Luminometer: The same plate reader that measures cell viability can be used to monitor glycolysis. No specialized instruments, plates or artificial medium are required.

Further Reading About Glucose-Glo™ Assay Development

  1. Zhou, W. et al. (2014) Self-immolative bioluminogenic quinone luciferins for NAD(P)H assays and reducing capacity-based cell viability assays. Chem BioChem 15, 670–5.
  2. Vidugiriene, J. et al. (2014) Bioluminescent cell-based NAD(P)/NAD(P)H assays for rapid dinucleotide measurement and inhibitor screening. Assay and Drug Development Technologies 12, 514–26.
  3. Leippe, D. et al. (2017) Bioluminescent assays for glucose and glutamine metabolism: High-throughput screening for changes in extracellular and intracellular metabolites. SLAS Discovery 22(4), 366–77.

Specifications

You are viewing: J6021 Change Configuration

What's in the box?

Item Part # SizeConcentration

Reductase

G884A 1 × 55μl6mg/ml

Reductase Substrate

G885A 1 × 55μl

Luciferin Detection Solution

J135A 1 × 5ml

NAD

J136A 1 × 30μl

Glucose

J138A 1 × 50μl

Glucose Dehydrogenase

J140A 1 × 200μl

SDS

Choose language:

Download SDSPDF (175 KB) – English (United States)

Certificate of Analysis

See all certificates for J6021

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Specifications

You are viewing: J6022 Change Configuration

What's in the box?

Item Part # SizeConcentration

Reductase

G884B 1 × 275μl6mg/ml

Reductase Substrate

G885B 1 × 275μl

Luciferin Detection Solution

J135B 1 × 50ml

NAD

J136B 1 × 275μl

Glucose

J138A 1 × 50μl

Glucose Dehydrogenase

J140B 2 × 1ml

SDS

Choose language:

Download SDSPDF (175 KB) – English (United States)

Certificate of Analysis

See all certificates for J6022

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-stephen

Stephen

UK