In this experiment, K562 cells were exposed to various doses of bortezomib for 16 hours.
Top Panel: Cells were harvested, washed, and labeled with Alexa Fluor® 488 (green fluorescence, PtdSer:AnxV binding) and 7-AAD (red fluorescence, membrane integrity) and analyzed by flow cytometry (10,000 events).
Bottom Panel: K562 cells (10,000/well) were exposed to serial dilutions of bortezomibin the presence of the RealTime-Glo™ Annexin V Apoptosis Assay reagent. Luminescence (PtdSer:AnxV binding) and fluorescence (membrane integrity) were recorded kinetically. The 16 hour incubation with bortezomib is shown.