We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

CellTiter-Fluor™ Cell Viability Assay

G6081

A Non-Lytic Fluorescence Assay That Measures the Relative Number of Viable Cells in Culture

  • Multiplex with many other luminescent assays
  • Normalizing data for live-cell numbers make results more comparable to other data

Size

Catalog number selected: G6080

$ 120.00 Your price: Log In

Add to Helix Add to Helix Add to Helix
CellTiter-Fluor™ Cell Viability Assay
10ml
$ 120.00
Your price: Log In
Change Configuration

The CellTiter-Fluor™ Cell Viability Assay is a non-lytic, single-reagent-addition fluorescence assay that measures the relative number of viable cells in a population. The assay is based on measurement of a conserved and constitutive protease activity within live cells and therefore serves as a biomarker of cell viability. The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (Gly-Phe-AFC). The substrate enters intact cells, where it is cleaved by the live-cell protease activity to generate a fluorescent signal proportional to the number of living cells. The live-cell protease becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium.

The CellTiter-Fluor™ Assay also can be used in a single-well, sequential, multiplex format with other downstream assay chemistries to normalize data by cell number. Data from the assay can serve as an internal control and allow identification of errors resulting from cell clumping or compound cytotoxicity. The assay is compatible with many Promega luminescence assays or spectrally distinct fluorescence assay methods, such as measuring caspase activation, reporter gene expression or orthogonal measures of viability.

 


CellTiter-Fluor Cell Viability Assay Chemistry

6995MA

Multiplexing CellTiter-Fluor and Caspase-Glo 3/7 Assays

6865MA.eps
Multiplex of CellTiter-Fluor™ Assay and Caspase-Glo® 3/7 Assay.

The CellTiter-Fluor™ Reagent was added to wells and viability measured after incubation for 30 minutes at 37°C. Caspase-Glo® 3/7 Reagent was added and luminescence measured after a 30-minute incubation (10,000 cells/well).

Protocols

Specifications

You are viewing: 10ml Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608A 1 x 10μl

Assay Buffer

G610A 1 x 10ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Store at –20°C.

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: 5 × 10ml Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608A 5 x 10μl

Assay Buffer

G610A 5 x 10ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Store at –20°C.

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: 2 × 50ml Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608B 2 x 50μl

Assay Buffer

G610B 2 x 50ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Store at –20°C.

Patents and Disclaimers

Patent Pending.

Resources

workflow-background

Small Molecule Drug Discovery Workflow

Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom