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CellTiter-Fluor™ Cell Viability Assay

A Non-Lytic Fluorescence Assay That Measures the Relative Number of Viable Cells in Culture

  • Multiplex with many other luminescent assays
  • Normalizing data for live-cell numbers make results more comparable to other data

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Catalog number selected: G6080

$ 123.00
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CellTiter-Fluor™ Cell Viability Assay
10ml
$ 123.00
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The CellTiter-Fluor™ Cell Viability Assay is a non-lytic, single-reagent-addition fluorescence assay that measures the relative number of viable cells in a population. The assay is based on measurement of a conserved and constitutive protease activity within live cells and therefore serves as a biomarker of cell viability. The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (Gly-Phe-AFC). The substrate enters intact cells, where it is cleaved by the live-cell protease activity to generate a fluorescent signal proportional to the number of living cells. The live-cell protease becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium.

The CellTiter-Fluor™ Assay also can be used in a single-well, sequential, multiplex format with other downstream assay chemistries to normalize data by cell number. Data from the assay can serve as an internal control and allow identification of errors resulting from cell clumping or compound cytotoxicity. The assay is compatible with many Promega luminescence assays or spectrally distinct fluorescence assay methods, such as measuring caspase activation, reporter gene expression or orthogonal measures of viability.

 


CellTiter-Fluor Cell Viability Assay Chemistry

6995MA

Multiplexing CellTiter-Fluor and Caspase-Glo 3/7 Assays

The CellTiter-Fluor™ Reagent was added to wells and viability measured after incubation for 30 minutes at 37°C. Caspase-Glo® 3/7 Reagent was added and luminescence measured after a 30-minute incubation (10,000 cells/well).

Specifications

You are viewing: G6080 Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608A 1 × 10μl

Assay Buffer

G610A 1 × 10ml

SDS

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Certificate of Analysis

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For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G6081 Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608A 5 × 10μl

Assay Buffer

G610A 5 × 10ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G6082 Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608B 2 × 50μl

Assay Buffer

G610B 2 × 50ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

Patent Pending.

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