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CellTiter-Glo® One Solution Assay

Luminescent Assay Measures Number of Viable Cells in Culture

  • Frozen, ready-to-use assay eliminates several protocol steps

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Catalog number selected: G8461

$ 454.00
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CellTiter-Glo® One Solution Assay
100ml
$ 454.00
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Cell number correlates with luminescent output.

Cell number correlates with luminescent output.

A direct relationship exists between luminescence measured with the CellTiter-Glo® One Solution Assay and the number of cells in culture over three orders of magnitude. Serial twofold dilutions of Jurkat cells were made in RPMI 1640 with 10% FBS and plated in three formats using automated dispensing equipment. Volumes plated were 100µl of cells per well in a 96-well plate (Panel A), 10µl of cells per well in low-volume 384-well plate (Panel B) and 4µl of cells per well in a 1536-well plate (Panel C). An equivalent volume of CellTiter-Glo® One Solution was dispensed into each well. Luminescence was recorded 10 minutes after addition of CellTiter-Glo® One Solution. There is a linear relationship (r2 > 0.99) between luminescent signal and cell number in each plate format.

Specifications

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

The CellTiter-Glo® One Solution Assay can also be stored at 4°C for 48 hours or at 22°C for 10 hours with ~10–12% loss of activity and can withstand two additional freeze-thaw cycles after the first thaw, with approximately 10% loss of activity with each additional freeze-thaw cycle.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Specifications

You are viewing: G8462 Change Configuration

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

The CellTiter-Glo® One Solution Assay can also be stored at 4°C for 48 hours or at 22°C for 10 hours with ~10–12% loss of activity and can withstand two additional freeze-thaw cycles after the first thaw, with approximately 10% loss of activity with each additional freeze-thaw cycle.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

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