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CellTiter-Glo® One Solution Assay

G8462
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Luminescent Assay Measures Number of Viable Cells in Culture

  • Frozen, ready-to-use assay eliminates several protocol steps

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Catalog number selected: G8461

$ 434.00
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CellTiter-Glo® One Solution Assay
100ml
$ 434.00
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The CellTiter-Glo® One Solution Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, which indicates the presence of metabolically active cells. This frozen, ready-to-use format is based on the original CellTiter-Glo® Luminescence Cell Viability Assay chemistry and eliminates the need to combine buffer with lyophilized substrate when preparing reagent. The CellTiter-Glo® Assay is designed for use with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS) in 96- to 1536-well format, and cell proliferation and cytotoxicity assays.

The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo® One Solution Assay generates a stable "glow-type" luminescent signal with a half-life of greater than three hours. This extended half-life eliminates the need for reagent injectors and provides flexibility for continuous or batch-mode processing of multiple plates.

No reagent preparation is required; simply thaw and "add-mix-measure". This format reduces the number of plate-handling steps. Data can be recorded 10 minutes after reagent addition, and the assay measures cells at numbers below the detection limits of standard colorimetric and fluorometric assays.

Volumes are convenient for HTS applications, and the assay can be used with various multiwell formats including 96-well, regular, or low volume 384- and 1536-well plates. Data can be recorded by luminometer or CCD camera imaging device.

The CellTiter-Glo assay provides a stable, luminescent signal with a half-life >3 hours, depending on cell type and culture medium used, and it can be used with other nonlytic compatible assay chemistries from Promega.

Notes

Cat.# G8461 is sufficient for 1,000 assays at 100µl/assay in 96-well plates or 4,000 assays at 25µl/assay in 384-well plates. Cat.# G8462 is sufficient for 5,000 assays at 100µl/assay in 96-well plates or 20,000 assays at 25µl/assay in 384-well plates.

Cell number correlates with luminescent output.

Cell number correlates with luminescent output.
Cell number correlates with luminescent output.

Specifications

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SDS

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Download SDSPDF (43 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

The CellTiter-Glo® One Solution Assay can also be stored at 4°C for 48 hours or at 22°C for 10 hours with ~10–12% loss of activity and can withstand two additional freeze-thaw cycles after the first thaw, with approximately 10% loss of activity with each additional freeze-thaw cycle.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Specifications

You are viewing: G8462 Change Configuration

SDS

Choose language:

Download SDSPDF (43 KB) – English (United States)

Certificate of Analysis

See all certificates for G8462

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

The CellTiter-Glo® One Solution Assay can also be stored at 4°C for 48 hours or at 22°C for 10 hours with ~10–12% loss of activity and can withstand two additional freeze-thaw cycles after the first thaw, with approximately 10% loss of activity with each additional freeze-thaw cycle.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

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