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MultiTox-Fluor Multiplex Cytotoxicity Assay

A Fluorescent Assay That Measures Both Cell Viability and Cytotoxicity

  • Assay normalizes data for cell number
  • Reduces false-positive and false-negative results

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Catalog number selected: G9200

$ 249.00
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MultiTox-Fluor Multiplex Cytotoxicity Assay
10ml
$ 249.00
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Measure the Number of Live and Dead Cells Simultaneously

The single-reagent-addition, homogeneous, fluorescent assay measures cell viability and cytotoxicity by detecting two distinct protease activities. The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant peptide substrate (GF-AFC Substrate). The substrate enters intact cells where it is cleaved to generate a fluorescent signal proportional to the number of living cells. This live-cell protease activity marker becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium. A second, cell-impermeant, fluorogenic peptide substrate (bis-AAF-R110 substrate) is used to measure dead-cell protease activity that has been released from cells that have lost membrane integrity. 

The homogeneous, "add-mix-measure" protocol eliminates parallel plate processing and reduces cell culture costs, and the ratio of live:dead cells is independent of cell number and normalizes data. Data normalization for cell number makes results more comparable well-to-well, plate-to-plate, day-to-day.

Complementary live- and dead-cell measures with independent chemistries serve as internal controls for each other, and multiplexing capability with most Promega bioluminescent cell-based apoptosis or genetic reporter assays results in more data per well.

The ratiometric response obtained from the MultiTox-Fluor Assay technology.

The ratiometric response obtained from the MultiTox-Fluor Assay technology.
The ratiometric response obtained from the MultiTox-Fluor Assay technology.

Notes

Cat # G9200 contains sufficient reagents for 100 assays at 100μl/assay in a 96-well format or 400 assays at 25μl/assay in a 384-well format. Cat # G9201 contains sufficient reagents for 500 assays at 100μl/assay in a 96-well format or 2,000 assays at 25μl/assay in a 384-well format. Cat # G9202 contains sufficient reagents for 1,000 assays at 100μl/assay in a 96-well format or 4,000 assays at 25μl/assay in a 384-well format.

Specifications

You are viewing: G9200 Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608A 1 × 10μl

bis-AAF-R110 Substrate

G609A 1 × 10μl

Assay Buffer

G610A 1 × 10ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Store at –20°C.

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G9201 Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608A 5 × 10μl

bis-AAF-R110 Substrate

G609A 5 × 10μl

Assay Buffer

G610A 5 × 10ml

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for G9201

Search

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Store at –20°C.

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G9202 Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608B 2 × 50μl

bis-AAF-R110 Substrate

G609B 2 × 50μl

Assay Buffer

G610B 2 × 50ml

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for G9202

Search

View more results

No results

Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Store at –20°C.

Patents and Disclaimers

Patent Pending.

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