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PLoS Pathogens 14, e1007057. Plasmodium parasite exploits host aquaporin-3 during liver stage malaria infection. 2018

Posfai, D., Sylvester, K., Reddy, A., Ganley, J.G., Wirth, J., Cullen, Q.E., Dave, T., Kato, N., Dave, S.S., and Derbyshire, E.R.

Notes: Researchers studied the liver stage of the Plasmodium parasite (which causes malaria), including RNA sequencing, parasite load and cell viability. (5035)

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PLos ONE 13, e0197350. Traditional and systems biology based drug discovery for the rare tumor syndrome neurofibromatosis type 2. 2018

Synodos for NF2 Consortium, Allaway, Rl, Angus, S.P., Beauchamp, R.L., Blakeley, J.O., Bott, M., Burns, S.S., Carlstedt, A., Chang, L.S., Chen, X., Clapp, D.W., Desouza, P.A., Erdin, S., Fernandez-Valle, C., Guinney, J., Gusella, J.F., Haggarty, S.J., Johnson, G.L., La Rosa, S., Morrison, H., Petrilli, A.M., Plotkin, S.R., Pratap, A., Ramesh, V., Sciaky, N., Stemmer-Rachamimov, A., Stuhlmiller, T.J., Talkowski, M.E., Welling, D.B., Yates, C.W., Zawistowski, J.S., and Zhao, W.N. 

Notes: Cell viability results were used to evaluate the effectiveness of agents against schwannoma and meningioma cell systems. (5028)

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Cell Chemical Biology 24(3), 281–292. Non-steroidal anti-inflammatory drugs are caspase inhibitors. 2017

Smith, C.E., Soti, S., Jones, T.A., Nakagawa, A., Xue, D., and Yin, H.

Notes: A novel target of non-steroidal anti-inflammatory drugs (NSAIDs) was identified using the Caspase-Glo® -1, Caspase-Glo® 3/7, and Caspase-Glo® 9 assays. The authors observed that an inhibition of caspase activity lead to decreased cell death and inflammation. The CellTiter-Fluor™ and CellTiter-Glo®  assays were using to monitor cell viability. (5163)

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Assay Drug Dev. Technol. 13, 456–65. Bioluminescent, nonlytic, real-time cell viability assay and use in inhibitor screening 2015

Duellman, S.J., Zhou, W., Meisenheimer, P., Vidugiris, G., Cali, J., Gautam, P., Wennerberg, K. and Vidugiriene, J.

Notes: The authors describe a homogeneous, nonlytic, bioluminescent assay that measures cell viability in real time. They monitored cell health for 72 hours from the same test samples, distinguished differential cell growth, and investigated drug mechanism of action by analyzing time- and dose-dependent drug effects. The real-time measurements enabled them to detect cell death immediately (>75% signal decrease within 15 minutes of digitonin addition), analyze drug potency versus efficacy, and identify cytostatic versus toxic drug effects.They then screened an oncology compound library (Z′ = 0.7) and identified compounds with varying activity at different time points. (4590)

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Assay Drug Dev. Technol. , epub ahead of print. Assessment of Compound Hepatotoxicity Using Human Plateable Cryopreserved Hepatocytes in a 1,536-well-plate Format. 2011

Moeller, T.A., Shukla, S.J. and Xia, M.

Notes: Here the authors describe development of an HTS cell viability assay protocol for use with cultured cyropreserved human primary hepatocytes. Cryopreserved hepatocytes for culturing were prepared as suspensions and dispensed at 2,000 or 4,000 cells/5µl/well in collagen I-coated 1,536-well plates. Cells were allowed to attach and then 23nl of each test compound was added in a dilution series from 2.8nM to 92µM, and cells incubated for 24 or 40 hours. Five microliters of CellTiter-Glo® Reagent was added and cells were incubated 30 minutes before reading the luminescent output. IC50 values for 12 compounds were determined; a summary of the protocol is provided in Table 1 of the paper. Cultured cryopreserved hepatocytes were assayed for function using the P450-Glo® CYP3A4 assay with the Luciferin-IPA substrate. (4182)

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Current Chemical Genomics 3, 33-41. In vitro viability and cytotoxicity testing and same-well multi-parametric combinations for high-throughput screening 2009

Niles, A.L., Moravec, R.A. and Riss, T.L.

Notes: The authors review the use of in vitro cytotoxicity testing in drug discovery to characterize the toxic potential of new chemical entities (nce) at the earliest stages of profiling. DOI: 10.2174/1875397300903010033 (4000)

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