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Caspase-Glo® 9 Assay Systems

A Luminescent Assay to Measure Caspase-9 Activity

  • Decreased assay time and less enzyme needed
  • Homogeneous “add-mix-measure” protocol amenable to automation
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Catalog number selected: G8210

$ 135.00 Your price: Log In

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Caspase-Glo® 9 Assay Systems
2.5ml
$ 135.00
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The Caspase-Glo® 9 Assay is a homogeneous luminescent assay that measures caspase-9 activity. The assay provides a proluminogenic caspase-9 substrate in a buffer system optimized for caspase activity, luciferase activity and cell lysis. The addition of a single Caspase-Glo® 9 Reagent in an "add-mix-read" format results in cell lysis, followed by caspase cleavage of the substrate and generation of a "glow-type" luminescent signal. The signal generated is proportional to the amount of caspase activity present. The Caspase-Glo® Reagent relies on the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant Luciferase), which generates the stable "glow-type" luminescent signal and improves performance across a wide range of assay conditions.

The system now includes a separate vial of a protease inhibitor, MG-132 Inhibitor, which may be used to reduce background, thus improving the performance of the Caspase-Glo® 9 Assay in cell-based applications.

Features and Benefits

  • The homogeneous "add-mix-read" protocol makes the assay easy to automate; simply add an equal volume of reagent to sample volume.
  • The low background luminescence results in excellent signal-to-noise ratios and superior sensitivity not achieved by other caspase formats, allowing assays to be performed in 96- or 384-well formats.
  • No sample preparation or manipulation required, and no extended incubation times are necessary as with fluorescent-based assays. Maximum sensitivity is achieved in as little as 0.5–1 hour.
  • The assay delivers excellent Z´ factors in cell and purified enzyme models.
  • The Caspase-Glo® 9 Assay uses a luminogenic substrate containing the LEHD sequence, which has been shown to be selective for caspase-9. The assay includes an optional proteasome inhibitor (MG-132), which when added to the Caspase-Glo® 9 Reagent significantly reduces nonspecific background in cell-based assays.
  • Multiplex with other cell-based assays from Promega.

Applications include caspase-9 activity determination, caspase-9 inhibition determination (IC50), and caspase-9 activation determination (EC50).

Notes

Using 100μl of Caspase-Glo® Reagent per assay in a 96-well format, Cat.# G8210 contains sufficient reagents for 25 assays; Cat.# G8211, 100 assays; Cat.# G8212, 1,000 assays, respectively. Using 25μl of Caspase-Glo® Reagent per assay in a 384-well format, Cat.# G8210 contains sufficient reagents for 100 assays; Cat.# G8211, 400 assays; Cat.# G8212, 4,000 assays, respectively.

Specifications

You are viewing: G8210 Change Configuration

What's in the box?

Item Part # Size

Caspase-Glo® 9 Substrate

G816C 1 × 1 bottle

Caspase-Glo® 9 Buffer

G822C 1 × 2.5ml

MG-132 Inhibitor

G932A 1 × 7.5μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: G8211 Change Configuration

What's in the box?

Item Part # Size

Caspase-Glo® 9 Substrate

G816A 1 × 1 bottle

Caspase-Glo® 9 Buffer

G822A 1 × 10ml

MG-132 Inhibitor

G932B 1 × 30μl

SDS

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Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: G8212 Change Configuration

What's in the box?

Item Part # Size

Caspase-Glo® 9 Substrate

G816B 1 × 1 bottle

Caspase-Glo® 9 Buffer

G822B 1 × 100ml

MG-132 Inhibitor

G932C 1 × 300μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

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