Notes: In this paper, the authors describe the development of an assay to monitor apoptosis using a novel bioluminescent method to detect annexin V binding to phosphatidylserine (PS) along with fluorescent detection of compromised membrane integrity. Assay development, characterization and benchmarking against established methods is demonstrated. (5180)

Notes: Hypoxic cancer cells constitute a major challenge in chemo- and radiotherapy. The researchers present evaluation of APD-CLD compounds that induce selective cell death in hypoxic cancer cells by impeding mitochondrial function. The RealTime-Glo™ Annexin V Apoptosis Assay, CellTiter-Blue® Cell Viability Assay, CellTox™ Green Real-Time Cytotoxicity Assay and GSH-Glo™ Glutathione Assay were used to assess mechanism of action. (5168)

Notes: Human pancreatic epithelial carcinoma cells were dispensed at 2,500 cells/well with 20µl of medium in 384-well 3D spheroid culture plates, the plates centrifuged and incubated for 1 day. After verifying spheroid formation, test or control compounds were added the cultured cells, incubated for 24 hours and 20µl of CellTiter-Glo® 3D Cell Viability Assay Reagent was added. Luminescence was measured after 30 minutes. BxPC-3-KRASG12V and BxPC-3-KRASWT cells were seeded into 384-well plates at a density of 2,500 cells/well in 20µl of medium as 3D or 2D cultures. After 24 hours, test compounds or vehicle are added followed by the RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay Reagent and luminescence monitored for 24 hours. (4971)