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Apoptosis Nov 29. , doi: 10.1007/s10495-018-1502-7. [Epub ahead of print]. A real-time, bioluminescent annexin V assay for the assessment of apoptosis. 2018

Kupcho, K., Shultz, J., Hurst, R., Hartnett, J., Zhou, W., Machleidt, T., Grailer, J., Worzella, T., Riss, T., Lazar, D., Cali, J.J. and Niles, A.

Notes: In this paper, the authors describe the development of an assay to monitor apoptosis using a novel bioluminescent method to detect annexin V binding to phosphatidylserine (PS) along with fluorescent detection of compromised membrane integrity. Assay development, characterization and benchmarking against established methods is demonstrated. (5180)

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Cell Chemical Biology 25, 1337–1349. APD-containing cyclolipodepsipeptides target mitochondrial function in hypoxic cancer cells. 2018

Jacobsen, K.M., Villadsen, N.L., Tørring, T., Nielsen, C.B., Salomón, T., Nielsen, M.M., Tsakos, M., Sibbersen, C., Scavenius, C., Nielsen, R., Christensen, E.I., Guerra, P.F., Bross, P., Pedersen, J.S., Enghild, J.J., Johannsen, M., Frøkiær, J., Overgaard, J., Horsman, M.R., Busk, M. and Poulsen, T.B.

Notes: Hypoxic cancer cells constitute a major challenge in chemo- and radiotherapy. The researchers present evaluation of APD-CLD compounds that induce selective cell death in hypoxic cancer cells by impeding mitochondrial function. The RealTime-Glo™ Annexin V Apoptosis Assay, CellTiter-Blue® Cell Viability Assay, CellTox™ Green Real-Time Cytotoxicity Assay and GSH-Glo™ Glutathione Assay were used to assess mechanism of action. (5168)

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Oncogene [Epub ahead of print]. A novel three-dimensional high-throughput screening approach identifies inducers of a mutant KRAS selective lethal phenotype. 2018

Kota, S., Hou, S., Guerrant, W., Madoux, F., Troutman, S., Fernandez-Vega, V., Alekseeva, N., Madala, N., Scampavia, L., Kissil, J. and Spicer, T.P.

Notes: Human pancreatic epithelial carcinoma cells were dispensed at 2,500 cells/well with 20µl of medium in 384-well 3D spheroid culture plates, the plates centrifuged and incubated for 1 day. After verifying spheroid formation, test or control compounds were added the cultured cells, incubated for 24 hours and 20µl of CellTiter-Glo® 3D Cell Viability Assay Reagent was added. Luminescence was measured after 30 minutes. BxPC-3-KRASG12V and BxPC-3-KRASWT cells were seeded into 384-well plates at a density of 2,500 cells/well in 20µl of medium as 3D or 2D cultures. After 24 hours, test compounds or vehicle are added followed by the RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay Reagent and luminescence monitored for 24 hours. (4971)

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