Kazusa Collection

Kazusa Genome Technology (KGT)

Kazusa Genome Technology (KGT) is an academic Japanese research institute dedicated to genome-scale studies and the function of individual genes. The Kazusa ORFeome Project was initiated to create a collection of human and mouse ORFs to enable the larger research community with a readily accessible, highly validated ORFeome. KGT is an active member of the global ORFeome Collaboration, and are experts in HUGE (Human Unidentified Gene-Encoded) large proteins (>4kb). Promega and KGT have established a partnership, allowing Promega customers access to Kazusa's coveted collection of experimentally validated clones.

Experimentally-Validated Collection

Kazusa understands the value of extensive validation, and provides this data to their customers for the HaloTag® ORF collection. Check out the representative data pieces below, which are available for nearly all HaloTag®-fusion ORFs before you order.

Fluorescent Microscopy demonstrating in situ labeling and visualization of HaloTag® fusions with HaloTag® TMR Ligand	Expression Validation by SDS-PAGE analysis of expressed HaloTag®-fusions in HEK293 cell lysates
Insert Size Confirmation through agarose gel electrophoresis	Sequence Validation of both the 5' and 3' end sequences by single-pass sequencing

Overview of the Collection

Discounts are available for bulk orders of multiple ORF clones (purified DNA).

• 10% discount by ordering 20-49 clones
• 20% discount by ordering more than 50 clones

Inquire at proteomics@promega.com before placing an order. Alternatively consider purchasing of an arrayed gene panel of HaloTag ORF clones as a bacterial glycerol stock.