Kazusa Genome Technology (KGT)
Kazusa Genome Technology (KGT) is an academic Japanese research institute dedicated to genome-scale studies and the function of individual genes. The Kazusa ORFeome Project was initiated to create a collection of human and mouse ORFs to enable the larger research community with a readily accessible, highly validated ORFeome. KGT is an active member of the global ORFeome Collaboration, and are experts in HUGE (Human Unidentified Gene-Encoded) large proteins (>4kb). Promega and KGT have established a partnership, allowing Promega customers access to Kazusa's coveted collection of experimentally validated clones.
Experimentally-Validated Collection
Kazusa understands the value of extensive validation, and provides this data to their customers for the HaloTag® ORF collection. Check out the representative data pieces below, which are available for nearly all HaloTag®-fusion ORFs before you order.
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Fluorescent Microscopy demonstrating in situ labeling and visualization of HaloTag® fusions with HaloTag® TMR Ligand
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Expression Validation by SDS-PAGE analysis of expressed HaloTag®-fusions in HEK293 cell lysates
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Insert Size Confirmation through agarose gel electrophoresis
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Sequence Validation of both the 5' and 3' end sequences by single-pass sequencing
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Overview of the Collection
| Features |
HaloTag® Collection
PID beginning with FHC |
Flexi® Collection
PID beginning with FXC |
| Size of Collection |
>9000 |
>6300 |
| Fusion Tag |
HaloTag® for protein purification, imaging and pull-downs |
Un-tagged |
| Validated Clones |
| Sequence Validated |
✓ 100% clones |
✓ |
| Insert Validated |
✓ 99.9% clones |
✓ 97.2% |
| Expression Validated |
✓ 99.2% clones |
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| Imaging Validated |
✓ 78% clones |
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| Format |
DNA |
DNA |
| Typical Delivery |
4-6 weeks |
4-6 weeks |
Discounts are available for bulk orders of multiple ORF clones (purified DNA).
- 10% discount by ordering 20-49 clones
- 20% discount by ordering more than 50 clones
Inquire at proteomics@promega.com before placing an order. Alternatively consider purchasing of an arrayed gene panel of HaloTag ORF clones as a bacterial glycerol stock.