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GoTaq® qPCR and RT-qPCR Systems

Convenient Master Mixes for Quantitative Real-Time PCR and RT-qPCR

  • Ready-to-use mix of optimized components for dye-based qPCR or RT-qPCR
  • Compatible with both fast and standard cycling methods
  • Resistant to a wide range of PCR inhibitors

Choose a system

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Catalog number selected: A6001

$ 290.00 Your price:

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GoTaq® qPCR and RT-qPCR Systems
qPCR/5ml
$ 290.00
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Simple, Sensitive, Dye-Based qPCR and RT-qPCR

The GoTaq® qPCR and RT-qPCR Systems are ready-to-use, 2X master mixes containing BRYT Green® Dye, a  fluorescent DNA binding dye with minimal PCR inhibition, providing maximum amplification efficiency and greater fluorescence enhancement than SYBR® Green I.

Rapid hot-start activation and processive enzymes make the  GoTaq® Systems compatible with most real-time PCR instruments using standard or fast cycling. The 1-Step and 2-Step RT-qPCR Systems also include GoScript™ Reverse Transcriptase, to enable efficient synthesis of first-strand cDNA in preparation for PCR.

Choose qPCR or RT-qPCR Master Mix Formats

GoTaq® qPCR Master Mix
GoTaq® 1-Step RT-qPCR System
GoTaq® 2-Step RT-qPCR System

Sensitivity and Specificity for qPCR

The GoTaq® qPCR Master Mix is a ready-to-use 2X master mix for real-time quantitative PCR. Combining GoTaq® Hot Start Polymerase, optimized buffer and the BRYT Green® Dye, the GoTaq® qPCR Master Mix provides robust real-time PCR with earlier quantification cycle values and broad-range detection. These qualities result in increased reliability, reproducibility and sensitivity for quantitative, real-time PCR.

Designed for use with any instrument capable of reading SYBR® Green I or FAM™ Dye, the GoTaq® qPCR Master Mix offers:

  • Sensitive detection for earlier quantitation of low- and high-copy targets.
  • Room-temperature setup,making it suitable for automation and high-throughput detection.
  • Compatibility with both fast and standard qPCR cycling methods.
Cat.#Size20µl Reactions10µl Reactions
A6001
5ml
500 reactions
1,000 reactions
A6002
25ml
2,500 reactions
5,000 reactions
GoTaq qPCR Master Mix provides high sensitivity in detecting a single-copy gene.
qPCR with single-copy detection. The GAPDH gene was amplified and detected from 10-fold serial dilutions (0.01ng to 100ng) of human genomic DNA. Inset shows the standard curve for the various dilutions (Slope=-3.2; R2=0.995). Kit yield and purity were measured by absorbance spectroscopy.

Reverse Transcription and qPCR in One Reaction

The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step real-time RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single reaction well. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. 

Other features provided by the 1-Step System include:

  • Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range. Detects down to femtogram (fg) amounts of input RNA.
  • Exceptional room-temperature setup, allowing easy automation and high-throughput detection.
Cat.#Size20µl Reactions10µl Reactions
A6020
5ml
500 reactions
1,000 reactions
10302TA_600px
Linear dynamic range from 100ng down to 10fg of human RNA template. Seven 10-fold dilutions of human total RNA was run using 200nM of each GAPDH transcript specific primer. The amplification curve shows 97% efficiency and an r2=0.999.

Two-Step Reverse Transcription and qPCR

The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.

The GoTaq® qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

 

Cat.#Size20µl Reactions10µl Reactions
A6010
5ml
50 × 20µl RT reactions
and 500 × 20µl qPCR reactions
50 × 20µl RT reactions
and 1,000 × 10µl
qPCR reactions
9017TA
Extended linear range. Dilutions of human reference total RNA were amplified with GoTaq® 2-Step RT-qPCR System looking at 5.8S expression. GoTaq® 2-Step system demonstrates a linear dynamic range over 9 orders of  magnitude with a high fluorescent signal. NTC reactions were all blank.

Performance Tested in qPCR with Many Sample Types and Instruments

We have generated experimental data using different systems, instruments, templates, samples and more. Just select the options below to narrow the selection.

 
 

Specifications

You are viewing: A6001 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

GoTaq® qPCR Master Mix, 2X

A600A 5 × 1ml

CXR Reference Dye

C541A 1 × 100μl View Product

Nuclease-Free Water

P119E 2 × 13ml View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

Specifications

You are viewing: A6002 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

GoTaq® qPCR Master Mix, 2X

A600A 25 × 1ml

CXR Reference Dye

C541A 5 × 100μl View Product

Nuclease-Free Water

P119E 10 × 13ml View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

Specifications

You are viewing: A6020 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 1 × 750μl25mM View Product

GoTaq® qPCR Master Mix, 2X

A600A 5 × 1ml

Nuclease-Free Water

P119E 2 × 13ml View Product

CXR Reference Dye

C541B 1 × 200μl30μM View Product

GoScript™ RT Mix for 1-Step RT-qPCR

M700A 1 × 225μl50X

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

Specifications

You are viewing: A6010 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 1 × 750μl25mM View Product

GoScript™ 5X Reaction Buffer

A500B 1 × 300μl View Product

GoScript™ Reverse Transcriptase

A501B 1 × 50μl

GoTaq® qPCR Master Mix, 2X

A600A 5 × 1ml

Oligo(dT)15 Primer

C110B 1 × 50μg500μg/ml View Product

PCR Nucleotide Mix

C114G 1 × 200μl10mM View Product

Random Primers

C118B 1 × 50μg View Product

CXR Reference Dye

C541A 1 × 100μl View Product

Recombinant RNasin® Ribonuclease Inhibitor

N251A 1 × 2,500u40u/μl

Nuclease-Free Water

P119E 2 × 13ml View Product

SDS

Choose language:

Certificate of Analysis

See all certificates for A6010 Search
View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

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