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qPCR Kits: GoTaq® qPCR and RT-qPCR Systems

Convenient Master Mixes for Real-Time qPCR and RT-qPCR

  • Ready-to-use mix of optimized components for dye-based qPCR or RT-qPCR
  • Compatible with both fast and standard cycling methods
  • Resistant to a wide range of PCR inhibitors

Choose a system

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Catalog number selected: A6001

$ 340.00
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qPCR Kits: GoTaq® qPCR and RT-qPCR Systems
qPCR/5ml
$ 340.00
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Simple, Sensitive, Dye-Based qPCR and RT-qPCR

The GoTaq® qPCR and RT-qPCR Systems are ready-to-use, 2X master mixes containing BRYT Green® Dye, a  fluorescent DNA binding dye with minimal PCR inhibition, providing maximum amplification efficiency and greater fluorescence enhancement than SYBR® Green I.

Rapid hot-start activation and processive enzymes make the  GoTaq® Systems compatible with most real-time PCR instruments using standard or fast cycling. The 1-Step and 2-Step RT-qPCR Systems also include GoScript™ Reverse Transcriptase, to enable efficient synthesis of first-strand cDNA in preparation for PCR.

Choose qPCR or RT-qPCR Master Mix Formats

GoTaq® qPCR Master Mix
GoTaq® 1-Step RT-qPCR System
GoTaq® 2-Step RT-qPCR System

Sensitivity and Specificity for qPCR

The GoTaq® qPCR Master Mix is a ready-to-use 2X master mix for real-time quantitative PCR. Combining GoTaq® Hot Start Polymerase, optimized buffer and the BRYT Green® Dye, the GoTaq® qPCR Master Mix provides robust real-time PCR with earlier quantification cycle values and broad-range detection. These qualities result in increased reliability, reproducibility and sensitivity for quantitative, real-time PCR.

Designed for use with any instrument capable of reading SYBR® Green I or FAM™ Dye, the GoTaq® qPCR Master Mix offers:

  • Sensitive detection for earlier quantitation of low- and high-copy targets.
  • Room-temperature setup,making it suitable for automation and high-throughput detection.
  • Compatibility with both fast and standard qPCR cycling methods.
View GoTaq® qPCR Master Mix Citations
Size Cat.# 10µl Reactions 20µl Reactions
A6001 5ml 1,000 reactions 500 reactions
A6002 25ml 5,000 reactions 2,500 reactions
qPCR master mix data
qPCR with single-copy detection. The GAPDH gene was amplified and detected from 10-fold serial dilutions (0.01ng to 100ng) of human genomic DNA. Inset shows the standard curve for the various dilutions (Slope=-3.2; R2=0.995). Kit yield and purity were measured by absorbance spectroscopy.

Reverse Transcription and qPCR in One Reaction

The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step real-time RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single reaction well. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.

Other features provided by the 1-Step System include:

  • Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range. Detects down to femtogram (fg) amounts of input RNA.
  • Exceptional room-temperature setup, allowing easy automation and high-throughput detection.
View GoTaq® 1-Step System Citations
Size Cat.# 10µl Reactions 20µl Reactions
A6020 5ml 1,000 reactions 500 reactions
A6020 1-Step Real-Time PCR & qPCR Kit
Linear dynamic range from 100ng down to 10fg of human RNA template. Seven 10-fold dilutions of human total RNA was run using 200nM of each GAPDH transcript specific primer. The amplification curve shows 97% efficiency and an r2=0.999.

Two-Step Reverse Transcription and qPCR

The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.

The GoTaq® qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

View GoTaq® 2-Step System Citations
Size Cat.# 10µl Reactions 20µl Reactions
A6010 5ml 50 × 20µl RT reactions and 1,000 × 10µl qPCR reactions 50 × 20µl RT reactions and 500 × 20µl qPCR reactions
A6010 2-Step Real-Time PCR & qPCR Kit
Extended linear range. Dilutions of human reference total RNA were amplified with GoTaq® 2-Step RT-qPCR System looking at 5.8S expression. GoTaq® 2-Step system demonstrates a linear dynamic range over 9 orders of  magnitude with a high fluorescent signal. NTC reactions were all blank.

Performance Tested in qPCR 

We have generated a large collection of data using different instruments, templates, and sample types. Visit the qPCR Data Selector to see the results generated using the GoTaq® qPCR systems.
View qPCR Data

Suitable for Different Organisms and Sample Types

DNA/RNA isolated from the following organisms and sample types has been used with GoTaq® qPCR Systems. DNA/RNA from organisms and sample types not listed may also be compatible—please enquire with Promega technical support for additional compatibility.

Organism Types:

  • Animal
  • Bacteria
  • Fungi
  • Human
  • Parasites
  • Viruses
  • Yeast

Sample Types:

  • Blood
  • Cells
  • Feces
  • FFPE
  • Food
  • Human Cerebrospinal Fluid (CSF)
  • Plants
  • Plasma
  • Saliva
  • Serum
  • Sputum
  • Swabs
  • Tissue
  • Urine

Compatible with these Instruments

For further details on instrument compatibility, please reference the appropriate Technical Manual. Instruments not listed may also be compatible—please enquire with Promega technical support for additional compatibility.

  • Applied Biosystems ABI PRISM® 7000 and 7700 Sequence Detection System
  • Applied Biosystems 7300 and 7900HT Real-Time PCR System
  • Applied Biosystems GeneAmp® 5700 Thermal Cycler
  • Applied Biosystems StepOne™ and StepOnePlus™ Real-Time PCR Systems
  • Applied Biosystems 7500 and 7500 FAST Real-Time PCR System
  • Applied Biosystems ViiA® 7 Real-Time PCR System
  • Applied Biosystems QuantStudio® Real Time PCR Systems
  • Bio-Rad CFX96 Real-Time PCR Detection System
  • Bio-Rad DNA Engine Opticon® and Opticon® 2 Real Time PCR Detection Systems
  • Bio-Rad iCycler® iQ™ and iQ™ 5 Real-Time PCR Detection System
  • Bio-Rad/MJ Research Chromo4™ Real-Time Detector
  • Bio-Rad MyiQ™ Real-Time PCR Detection System
  • Cepheid SmartCycler® System
  • Corbett Rotor-Gene™ 3000 and 6000 Real-Time Rotary Analyzer
  • Eppendorf Mastercycler® ep realplex Real-Time PCR System
  • Roche LightCycler® 480 Real-Time PCR System
  • Stratagene Mx3000P® and Mx3005P® Real-Time PCR Systems
  • Stratagene Mx4000® Multiplex Quantitative PCR System

Specifications

You are viewing: A6001 Change Configuration

What's in the box?

Item Part # Size Available Separately

GoTaq® qPCR Master Mix, 2X

A600A 5 × 1ml

CXR Reference Dye

C541A 1 × 100μl View Product

Nuclease-Free Water

P119E 2 × 13ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

Specifications

You are viewing: A6002 Change Configuration

What's in the box?

Item Part # Size Available Separately

GoTaq® qPCR Master Mix, 2X

A600A 25 × 1ml

CXR Reference Dye

C541A 5 × 100μl View Product

Nuclease-Free Water

P119E 10 × 13ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

Specifications

You are viewing: A6020 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

MgCl2

A351B 1 × 750μl 25mM View Product

GoTaq® qPCR Master Mix, 2X

A600A 5 × 1ml

Nuclease-Free Water

P119E 2 × 13ml View Product

CXR Reference Dye

C541B 1 × 200μl 30μM View Product

GoScript™ RT Mix for 1-Step RT-qPCR

M700A 1 × 225μl 50X

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

Specifications

You are viewing: A6010 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

MgCl2

A351B 1 × 750μl 25mM View Product

GoScript™ 5X Reaction Buffer

A500B 1 × 300μl View Product

GoScript™ Reverse Transcriptase

A501B 1 × 50μl

GoTaq® qPCR Master Mix, 2X

A600A 5 × 1ml

Oligo(dT)15 Primer

C110B 1 × 50μg 500μg/ml View Product

PCR Nucleotide Mix

C114G 1 × 200μl 10mM View Product

Random Primers

C118B 1 × 50μg View Product

CXR Reference Dye

C541A 1 × 100μl View Product

Recombinant RNasin® Ribonuclease Inhibitor

N251A 1 × 2,500u 40u/μl View Product

Nuclease-Free Water

P119E 2 × 13ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

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