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GoTaq® Probe 1-Step RT-qPCR System Technical Manual

Instructions for Use of Product(s)
A6120, A6121

Literature # TM379

The GoTaq® Probe 1-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in single-step real-time amplification reactions.

The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.

The GoTaq® Probe qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). This master mix does not contain a reference dye; however, a separate tube of carboxy-X-rhodamine (CXR) reference dye is included with this system, allowing you to add reference dye to amplification reactions if desired.

Summary of Changes
The following changes were made to the 4/20 revision of this document:

  1. Product disclaimer text pertaining to Cat.# A6120, A6121 in Section 2, Product Components and Storage Conditions, was updated.
  2. Expired patent text was removed.

Revised 4/20.

Experienced User Protocols