Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

GoTaq® 1-Step RT-qPCR System Technical Manual

Instructions for Use of Product(s)

Literature # TM355

GoTaq® 1-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol. The GoTaq® 1-Step RT-qPCR System combines the benefits of GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix for efficient,sensitive and linear one-step RT-qPCR quantification over a wide range of RNA template inputs.
The GoTaq® 1-Step RT-qPCR System contains the proprietary fluorescent DNA-binding dye, BRYT Green® dye, that exhibits greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA), than does SYBR® Green I.

Summary of Changes
The following change was made to the 12/18 revision of this document:
1. The product size was updated to reflect volume provided.
2. Incorporated other general updates.

Printed in USA. Revised 12/18

Experienced User Protocols