GoTaq® 1-Step RT-qPCR System Technical Manual

Instructions for Use of Product(s)

Literature # TM355

GoTaq® 1-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol. The GoTaq® 1-Step RT-qPCR System combines the benefits of GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix for efficient,sensitive and linear one-step RT-qPCR quantification over a wide range of RNA template inputs.
The GoTaq® 1-Step RT-qPCR System contains the proprietary fluorescent DNA-binding dye, BRYT Green® dye, that exhibits greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA), than does SYBR® Green I.

Summary of Changes
The following change was made to the 8/23 revision of this document:
1. Updated Sections 2 and 7.
2. Replaced cover image and document font.
3. Updated patent statements.
4. Made minor text edits.

Revised 8/23.

Experienced User Protocols