GoTaq® Probe 2-Step RT-qPCR System Technical Manual
Instructions for Use of Product(s)
Literature # TM380
The GoTaq® Probe 2-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system protocol facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix.
The GoScript™ Reverse Transcription System includes an optimized reaction buffer and reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product may be added directly to downstream qPCR amplification reactions.
The GoTaq® Probe qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). The master mix does not contain a reference dye; however, a separate tube of carboxy-X-rhodamine (CXR) reference dye is included with this system, allowing you to add reference dye to amplification reactions if desired.
Summary of Changes
The Storage Conditions were updated in the 9/18 revision of this document.
Printed in USA. Revised 9/18.