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Glucose-Glo™ Assay Technical Manual

Instructions for Use of Product(s)
J6021, J6022

Literature # TM494

The Glucose-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glucose in biological samples. The assay couples glucose oxidation and NADH production with a bioluminescent NADH detection system. Glucose dehydrogenase uses glucose and NAD+ to produce NADH. In the presence of NADH a pro-luciferin Reductase Substrate is converted by Reductase to luciferin, which is used in a luciferase reaction to produce light. 

The luminescent signal is proportional to the amount of glucose in the sample and increases until all glucose is consumed, at which point a stable luminescent signal is achieved.

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