Glutamate-Glo™ Assay Technical Manual
Literature # TM495
The Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamate in biological samples. The assay couples glutamate oxidation and NADH production with a bioluminescent NADH detection system. Glutamate dehydrogenase uses glutamate and NAD+ to produce α-ketoglutarate and NADH. In the presence of NADH a pro-luciferin Reductase Substrate is converted by Reductase to luciferin that is then used by Ultra-Glo™ Recombinant Luciferase to produce light.
The luminescent signal is proportional to the amount of glutamate in the sample and increases until all glutamate is consumed, at which point a stable luminescent signal is achieved.