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Glutamine/Glutamate-Glo™ Assay Technical Manual

Instructions for Use of Product(s)
J8021, J8022

Literature # TM496

The Glutamine/Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamine and glutamate in biological samples. In this two-step assay, glutamine is first converted to glutamate by glutaminase, followed by glutamate oxidation and NADH production coupled with a bioluminescent NADH detection system. In the presence of NADH, a pro-luciferin Reductase Substrate is converted by Reductase to luciferin, which is used in a luciferase reaction to generate light. 

The luminescent signal is proportional to the amount of glutamate and increases until all glutamate is consumed, at which point a stable luminescent signal is achieved.

Summary of Change
The following change was made to the 8/19 revision of this document:
Updated data in Table 7.

Printed in USA. Revised 8/19.