Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Product Availability Update

Promega Manufacturing and Delivery Systems continue to be fully operational during the COVID-19 outbreak. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Learn more

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Glutamine/Glutamate-Glo™ Assay Technical Manual

Instructions for Use of Product(s)
J8021, J8022

Literature # TM496

The Glutamine/Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamine and glutamate in biological samples. In this two-step assay, glutamine is first converted to glutamate by glutaminase, followed by glutamate oxidation and NADH production coupled with a bioluminescent NADH detection system. In the presence of NADH, a pro-luciferin Reductase Substrate is converted by Reductase to luciferin, which is used in a luciferase reaction to generate light. 

The luminescent signal is proportional to the amount of glutamate and increases until all glutamate is consumed, at which point a stable luminescent signal is achieved.

Summary of Change
The following change was made to the 8/19 revision of this document:
Updated data in Table 7.

Printed in USA. Revised 8/19.