The lipid accumulation in differentiating adipocytes was monitored with Oil Red O staining and the Triglyceride-Glo™ Assay. Parallel plates of 3T3L1-MBX fibroblasts were differentiated following standard protocol.
At days 5, 12, 14 and 21 (Panels A–D, respectively) the parallel plates were either stained with Oil Red O or assayed with the Triglyceride-Glo™ Assay for total glycerol content. The Triglyceride-Glo™ Assay quantitatively measures triglycerides levels in differentiated adipocytes.
The simple sample preparation protocol includes only a detergent lysis step and sample dilution prior to triglyceride quantification. No organic extraction or extreme heating steps are required.