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Reverse Transcription System

A0002

Convenient System for Reverse Transcription Using AMV Reverse Transcriptase

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Catalog number selected: A3500

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Reverse Transcription System
100 reactions
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The Reverse Transcription System provides reagents to efficiently reverse transcribe RNA into cDNA in 15 minutes. The cDNA prepared from each reaction using this system may be used directly in multiple PCR amplifications using Taq DNA polymerase. The AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly(A)+ RNA. Both Oligo(dT)15 and Random Primers are included, allowing cDNA synthesis from virtually any RNA source. The system contains sufficient reagents for 100 cDNA synthesis reactions, processing 1μg of RNA per reaction. Each cDNA synthesis reaction may be divided and used in up to 20 separate PCR amplifications. A polyadenylated 1.2kb RNA transcript is provided as a control template for cDNA synthesis.


  • Speed: Efficiently reverse transcribe poly(A)+ mRNA or total RNA in 15 minutes.
  • Convenience: PCR-compatible components are provided in optimized volumes for 100 reactions.
  • Positive Controls: A polyadenylated RNA transcript is provided to help troubleshoot RT-PCR parameters.

Protocols

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351H 1 x 1.2ml25mM View Product

Reverse Transcription 10X Buffer

A356A 1 x 1.4ml View Product

Oligo(dT)15 Primer

C110B 1 x 50μg500μg/ml View Product

dNTP Mix

C114B 1 x 320μl10mM

Random Primers

C118B 1 x 50μg View Product

1.2kb Kanamycin Positive Control RNA

C138A 1 x 5μg0.5mg/ml View Product

AMV Reverse Transcriptase (HC)

M900B 1 x 1,500u View Product

Recombinant RNasin® Ribonuclease Inhibitor

N251A 1 x 2,500u

Nuclease-Free Water

P119E 1 x 13ml View Product

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Resources

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RNA Analysis Workflow

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