Nucleic Acid Amplification
After you've purified and quantitated your nucleic acid, the next step is amplification. PCR is perhaps the most ubiquitous assay in molecular biology—something we do every day. Each type of PCR has different applications and comes with its own unique challenges. Which one is right for your project?
Introduction to PCR
Get a crash course in PCR basics and troubleshooting.
Top Ten Tips for Optimizing PCR
Your PCR run can almost always be better. Optimizing your protocol and following these tips could save you time, energy and the frustration of having to start over.
Dealing with PCR Inhibitors
Inhibitors, such as salts and detergents, can easily ruin your day. Learn how to avoid them in this blog post.
Is qPCR For You?
Find out if real-time qPCR could be the application you're looking for with this article full of videos and different methods to consider.
qPCR: The Very Basics
What's the difference between traditional endpoint PCR and real-time qPCR? This blog has the answers.
Introduction to Real-Time PCR
Check out this overview of basic qPCR principles, assay design and analysis.