Before you can analyze your recombinant protein of interest, you’ll need to purify it from your expression medium and other macromolecules. Affinity tags are one of the main strategies used to purify proteins and antibodies, and there are many tag systems to choose from. Check out these resources to help you choose the best affinity tag for your project.
Intro to Protein Tags for Purification
Affinity tags are a selective and versatile way to purify your protein of interest. Use this introductory guide to learn about what tagging systems are available and how they work.
What do you get when you combine a chain of histidine residues and nickel ions? One of the most widely used protein tagging systems. Learn more about polyhistidine tags.
Tags for Covalent Immobilization
Purify your protein by pulling it right out of solution. Read more in this scientific article.