The core of proteomics research is understanding how proteins function and interact with other macromolecules. There are multiple techniques which you can select from, but some are better than others to address your research question.
Proteins are dynamic and complex. Tagging your proteins can be a great way to visualize and analyze their functions and interactions.
With so many options for protein tagging, it can be hard to know where to start. Read this article to discover what protein tags are available and which you should choose for your experiment.
Learn about the HaloTag® protein, designed for use in multiple protein imaging applications.
Read more about about how HiBiT’s uniquely small size and versatility can help you overcome tagging challenges.
Learn why brighter isn’t always better when it comes to tagging.
Detecting Protein Interactions
Understanding how proteins interact and bind with macromolecules unveils their role in diseases and other biological processes.
Learn how to harness bioluminescent resonance energy transfer (BRET) technology in studying protein:protein interactions
Resonance Energy Transfer
Achieve a better understanding of bioluminescence resonance energy transfer (BRET) technology.
Complementation Reporter Systems
See how NanoBiT® technology can be used to improve your protein interaction measurements.
Learn how protein:DNA interactions can be studied and analyzed without the use of antibodies.
Live-Cell Protein:Protein Interactions
What are the differences and similarities between NanoBiT® and NanoBRET™? Read more on how to use these tools to investigate protein:protein interactions and determine which one is best for your needs.
Validating Protein Interactions
Learn how copurification pull-down assays can help you confirm BRET in a protein interaction.
ELISA protocols can be daunting. Watch the video to build your confidence in tackling this frequently used technique.
Optimizing Western Blots
Avoid common mistakes while saving time and effort with these tips for performing Western blots.
Watch this animation demonstrating the advantages of the Lumit™ system over other immunoassays.
One of the most powerful protein detection techniques is mass spectrometry. You can use this method to detect and quantify numerous protein properties.
Bottom-Up Proteomics Tips
Find resources on optimizing your bottom-up proteomics workflow.
Sample Prep for Mass Spec
New to mass spec? Learn why spending a little bit of time optimizing your sample prep steps will lead to better results in the end.
What to Expect During Mass Spectrometry
Avoid common pitfalls of mass spec sample prep when planning your next experiment.